嗜卷书虱抗气调品系的选育及其适合度研究

Studies on the resistance of Liposcelis bostrychophila to controlled atmosphere and its ecological fitness

于恒温条件下以人工饲料饲养的嗜卷书虱Liposcelisbostrychophila ,在 35%CO2 和 1%O2组配的气调环境中以 50 %左右的选择压力处理 ,以选育其抗气调性。至 30代获得抗性品系 (CA R) ,抗性倍数达 5 6倍 ,且有继续增强的基因潜能。该抗性品系对气调的抗性不太稳定 ,在无选择压力的情况下经室内 5代饲养 ,抗性衰退了 6 3 2 %。CA R品系与敏感品系在无气调选择压力的条件下相比具有一定程度的繁殖不利性 ;但没有发现CA R品系在生长发育特性上的不利性。以净增殖率来衡量 ,CA R品系相对于敏感品系具有 0 39的适合度。

Adult population of the psocid, Liposcelis bostrychophila, was reared with the artificial diet and exposed to the controlled atmosphere (CA) containing 35% CO 2, 1% O 2 and 64% N 2 at 28?℃ for 30 successive generations in order to select a strain resistant to the controlled atmosphere (CA\|R). Based on LT 50 values, the resistance factor (RF) had increased 5 6\|folds at 30th generation and it seemed to have the potential of further development. Removal of selection pressure revealed that the resistance of CA\|R was unstable and RF decreased by 63 2% after 5 successive generations. The effect of CA resistance on ecological fitness in the psocid was evaluated under CA\|free condition. The results showed that the CA\|R strain had no unfavorableness in development but obvious disadvantage in reproduction with a fitness value of only 0 39 relative to that of the strain sensitive to CA in terms of net reproductive rate (R 0).