纳米硫化镉对人肾小球系膜细胞氧化损伤及NF-κB蛋白表达的影响

Effects of nano-cadmium sulfide on oxidative damages and NF-κB expression in human mesangial cells

目的探讨纳米硫化镉（nano-CdS）对人肾小球系膜细胞（HMC）的氧化损伤及其机制。方法将对数生长期的HMC细胞分为对照组（0μg/ml）和nano-CdS组（5～80μg/ml）,用MTT实验观察nano-CdS对细胞存活率的影响,采用荧光探针技术检测细胞内的活性氧（ROS）,采用蛋白质印迹法观察NF-κB蛋白的表达。结果与对照组比较,nano-CdS组细胞存活率降低,ROS含量升高,NF-κB的蛋白表达增加,差异均有统计学意义（P〈0.05）。结论 nano-CdS可导致HMC细胞氧化损伤。

Objective To investigate the cytotoxicity of nano-cadmium sulfide（nano-CdS） on human glomerular mesangial cells（HMC）. Methods After HMC cells were exposed to different concentrations of nano-CdS（5-80 μg/ml） for 24 h, cell viability were observed by MTT assay. The content of ROS and level of NF-κB protein in cells were determined by fluorescence probe and Western blotting individually. Results Compared with the control group, the viability of HMC cells was decreased with increasing concentrations of nano-CdS（P〈0.05）. After exposed to nano-CdS for 24 h, as the concentration increased, the content of ROS and NF-κB protein was enhanced（P〈0.05）. Conclusion Cell toxicity of nano-CdS may be due to the mechanism involving antioxidant system damage.