湖北海棠阶段转变过程中几种同工酶变化研究

Dynamic of Several Isozymes of Malus hupenhensis in Phase Change

采用聚丙烯酰胺凝胶电泳法 ,研究了湖北海棠 1年生实生苗 (S1 )、4年生 (S4)、1 4年生 (S1 4 )实生树及 4年生成年芽嫁接树 (G4)不同高度叶片的过氧化物酶 (POX)、细胞色素氧化酶 (COD)、酯酶 (EST)、乙醇脱氢酶 (ADH)同工酶的变化。结果表明 :多年生实生树随着高度的增加 ,POXRf 0 .56、0 60 ,CODRf0 .50 ,ESTRf0 .0 9、0 1 5、0 75、0 77,ADHRf0 .76等几条酶带呈现差异 ,出现了童区和成年区各自特有的同工酶带 ,随高度变化童区特征酶带消失或成年区特征酶带出现的高度均保持在 1 50～ 2 0 0cm之间 ,同当年植株开花最低位置相同 ,S4、S1 4 童区与S1 同工酶谱带完全一致 ,S4、S1 4 成年区与G4同工酶谱基本保持一致 ,G4同工酶谱并未随高度增加而发生变化。四种同工酶可以作为阶段转变的指标。S4、S1 4 童区与成年区谱带的差异是高度的结果 ,高度是湖北海棠阶段转变的重要因子。

The height_associated dyamics of isozymes of peroxidase (POX), esterase (EST), cytochrome oxidase (COD) and alcohol dehydrogenase (ADH) in leaves were studied by polyacrylamide gel electrophoresis (PAGE) in 1,4,14_year_old seedlings and 4_year_old grafted plant, which grafted by adult_phase bud of Malus hupenhensis. The results showed that, the observability of some bands, such as POX Rf 0.56, 0.60, COD Rf 0.50, EST Rf 0.09, 0.15, 0.75, 0.77, ADH Rf 0.76, were age variable. Some were found only in adult_phase leaves, and others were unique to juvenile_phase leaves. The height from 150 to 200 cm, where adult_ or juvenile_associated isozymes disappeared or appeared was the lowest flowering height. 1_year_old seedling displayed patterns that were identical to juvenile zone of seedlings perennial. Grafted plant has essential bands of adult zone of seedlings perennial. The study disclosed that plant height may be one of the most important factors that govern phase change of Malus hupenhensis. The four isozymes could be served as markers to assess characterization of phase change of perential plants.