醛固酮促进心室成纤维细胞增殖作用

THE EFFECT OF ALDOSTERONE OF PROMOTION ON PROLIFERATION OF VENTRICULAR FIBROBLASTS

探讨醛固酮促进心室成纤维细胞增殖作用。方法 :采用 [3H] TdR掺入率测定和RT PCR。结果 :醛固酮 (1× 10 -9～ 1× 10 -6)mol/L能明显促进心室成纤维细胞的 [3H] TdR掺入率 ,且呈剂量依赖方式 ;醛固酮孵育 15min ,可见c fosmRNA的表达 ,至 1h表达达高峰 ,以后逐渐降低 ,醛固酮受体拮抗剂螺旋内酯能阻断醛固酮的作用。结论 :醛固酮能促进心室成纤维细胞增殖 。

Aim:To study the effect of promoting aldosterone on proliferation of ventricular fibroblasts. Methods: Assay of [ 3H] TdR incorporation rate and RT PCR were used.Results: Aldosterone could promote [ 3H] TdR incoporation of ventricular fibrolasts,the effective dose of aldosterone was among (1×10 -9 ～1×10 -6 )mol/L,and had dose dependent manner,the c fos gene was expressed after stimulated by aldosterone for 15 min,and studied the highest in 1 h,then reduced later.Spironolactone,aldosterone receptor antagonist could block the effect of aldosterone.Conclusion: Aldosterone promotes the proliferation of ventricular fibroblasts,mediated by aldosterone receptor.