Studies on RNA Transcription Activity in Interphase Nuclei in Physarum polycephalum

多头绒泡菌间期细胞核中RNA的转录状况(英文)

Using BrUTP incorporation and statistical analysis, the authors investigated RNA transcription in the interphase nuclei in Physarum polycephalum Schw. The rRNA synthesis in the nucleolus continued actively throughout the interphase, while in nucleoplasm hnRNA was transcribed at law levels in S phase and actively in G(2) phase. The rate of nucleoplasm RNA transcription was doubled between early S phase and late S phase, and a 5-6 folds increase was detected between the early S phase and G(2) phase.

利用BrUTP免疫标记技术 ,研究了多头绒泡菌 (PhysarumpolycephalumSchw .)间期细胞核中RNA的转录状况。结果表明 :在整个间期核仁中的rRNA都在活跃转录 ;核质中hnRNA的转录呈逐渐上升趋势 ,早S期转录水平很低 ,晚S期转录活性升高 1倍 ,G2 期转录达到最高水平 ;整个间期核质中RNA的转录水平增加了 5～