节节麦的酯酶同工酶分析

Diversity of esterase isozyme in Aegilops tauschii Cosson

对 30份不同来源的节节麦进行 4个时期的酯酶同工酶分析。结果表明 :不同来源节节麦的酯酶同工酶存在较大差异 ,共分成 1 5种基本类型。我国黄河流域的 1 0份节节麦被划分为 2个基本类型 ,但二者关系极为相近 ;新疆节节麦与之有一定差异 ,但在相似系数≤ 0 .82 0时可视为一类。所有材料在 4个时期之间没有出现一个完全相同的酶带类型 ,说明酯酶同工酶随发育时期而不断变化。

The esterase isozyme of 30 accessions of Aegilops tauschii were studied by means of polyarylamide gel electrophoresis. The results showed significant difference of esterase in all of the four stages i.e. seeding, Shooting, flag leaf and young ear, which patterns can be divided into 15 types. Ten accessions from middle reaches of the Yellow River belonged to two patterns with a similarity coefficient 0 984. Two accessions of Xinjiang belonged to one pattern and was different from that of middle reaches of Yellow River. No same esterase isozyme has been found in the four stages. It showed that esterase isozyme related to growing and development of plants.