整合素β1在大鼠髁突软骨力学适应性改建过程中的表达

Expression of integrin β1 in endochondral remodeling of rats

目的研究整合素β1(Itgβ1)在大鼠髁突软骨力学适应性改建过程中的表达变化,为进一步探索髁突软骨细胞的力学—生物学信号转导提供实验依据。方法 60只Sprague-Dawley大鼠随机分为实验组与对照组,实验组全天佩戴下颌前伸装置。分别于1、3、7、14、21、28 d取双侧髁突,检测Itgβ1在髁突软骨细胞中的表达情况,测量结果以免疫组织化学评分(IHS)表示。结果 Itgβ1表达量在整个实验周期中均明显高于对照组,且实验组Itgβ1表达量随时间呈现规律性变化并与髁突改建过程相应。结论 Itgβ1在大鼠下颌功能前伸后的髁突软骨力学适应性改建过程中发挥重要作用。

Objective This study aims to investigate the expression of integrin β1（Itgβ1） in endochondral remodeling of rats and provide experiment basis to the regulation mechanism of the mandibular condylar cartilage. Methods A total of 60 male Sprague-Dawley rats were randomly divided into experimental and control groups. In the experimental group, inclined bite plate was cemented to the upper central incisors of the rats for 1 d. Five rats were executed in each group after 1, 3, 7, 14, 21, and 28 d. The specimens of the bilateral temporomandibular joints were subjected to hematoxylin–eosin staining and immunohistochemistry against Itgβ1. Data were presented as immunohistochemical scores（IHS）. Results The IHS of Itgβ1 inthe experimental group was signifi cantly higher than that inthe control group, whereasthe changes were normal. Conclusion Itgβ1 has a crucial function in the reconstruction of the condylar cartilage during mandibular forward positioning.