腮腺主导管结扎后再通的组织学变化

Histological changes of the parotid gland following release from duct ligation

目的观察主导管结扎后再通腮腺的组织学变化。方法将Wistar大鼠右侧腮腺主导管结扎7 d,然后松开结扎使主导管再通,于再通后0、1、3、5、7、14、21 d获取腮腺标本,应用苏木精-伊红(HE)染色和形态计量方法,观察再通后腺体的组织学变化。结果主导管结扎7 d时腺体明显萎缩;于再通后1 d开始,腺泡体积分数逐渐增多,导管和间质体积分数逐渐减少(P<0.05);再通14 d后,腺泡、导管和间质所占体积分数均与正常对照组差异无统计学意义(P>0.05)。结论主导管结扎7 d后再通,萎缩的腺体能够恢复正常。

Objective The histological changes of the parotid gland were investigated following release from duct ligation. Methods The right parotid ducts of Wistar rats were ligated for 7 days and then reopened. The specimens were harvested at days 0, 1, 3, 5, 7, 14, and 21. The histological changes of the gland were investigated by hematoxylineosin（HE） staining and morphology measurement. Results The parotid gland atrophied signifi cantly after duct ligation for 7 days. From day 1 after duct reopening, the acinar volume fraction continued to increase signifi cantly; both the duct and mesenchymal volume fraction continued to decrease signifi cantly（P〈0.05）. After 14 days of regeneration, no signifi cant differences in the volume fractions of acinas, ducts, and mesenchyma were observed compared with the normal control group（P〉0.05）. Conclusion The atrophied parotid gland regenerated to normal after 7 days of duct ligation.