比较两种方法检测血清高尔基体膜蛋白-73诊断肝细胞肝癌的价值

Comparison of two methods to detect serum GP73 for the diagnosis of hepatocelluar carcinoma

目的 探讨血清高尔基体膜蛋白-73（GP73）对肝细胞肝癌的诊断价值以及Westem blot和Elisa 2种检测方法的优劣.方法 收集人外周血血清120例,其中肝细胞肝癌、乙型病毒性肝炎、乙型肝炎肝硬化、健康志愿者各30例.应用Western blot方法和Elisa方法检测各组血清GP73的表达水平,计算并比较诊断肝细胞肝癌的受试者工作特征曲线下面积、灵敏度、特异度,并与应用电化学发光法检测的血清甲胎蛋白（AFP）进行比较.结果 Western blot和Elisa 2种方法检测肝细胞肝癌患者血清GP73的表达水平均高于其他各组,差异有统计学意义（P＜0.05）;Western blot方法和Elisa方法检测GP73诊断肝细胞肝癌的受试者工作特征曲线下面积、灵敏度、特异度均高于AFP,差异有统计学意义（P＜0.05）;与Westemblot方法相比,Elisa方法检测的GP73诊断HCC的灵敏度及特异度差异无统计学意义（P＞0.05）.结论 Western blot和Elisa 2种方法均可有效地检测人体外周血清中GP73的表达水平,但Elisa方法操作相对简单、快速,可作为血清中GP73表达水平检测的首选方法;血清GP73检测肝细胞肝癌的诊断价值优于AFP,有望成为肝细胞肝癌诊断的血清肿瘤标志物.

Objective To investigate the level of serum Golgi protein-73 （GP73） in hepatocellular carcinoma （HCC） by Western blotting and Elisa.Methods Peripheral serum samples were collected from 120 people including 30 HCC patients,30 cirrhotic patients,30 hepatitis B patients and 30 healthy volunteers.Then,the levels of serum GP73 were detected by Western blotting and Elisa,while the area under the receiver operating characteristic curve,sensitivity and specificity were calculated.Meanwhile,the level of AFP was detected by electrochemiluminescence.Results HCC patients presented higher levels of serum GP73 than others after examination by Western blotting and Elisa （P 〈 0.05）.Both methods showed higher area under the receiver operating characteristic curve,sensitivity and specificity than AFP when detecting of the level of GP73 for HCC diagnosis （P 〈 0.05）.No statistically significant difference was found as to sensitivity and specificity between Western blotting and ELISA （P 〉 0.05）.Conclusion Both Western blot and Elisa can be adopted to detect the level of human peripheral serum GP73 for HCC diagnosis.However,Elisa is simple and fast and can be the preferred method for detecting the level of serum GP73.Compared with AFP,serum GP73 detection presented higher AUC,sensitivity and specificity,indicating the potential role of serum GP73 as a tumor marker for the diagnosis of hepatocellular carcinoma.