微波辅助提取牡丹雄蕊总生物碱工艺研究

The microwave-assisted extraction technology of total alkaloids from peony stamens

采用微波辅助法提取牡丹雄蕊中的总生物碱。考察了料液比、浸润时间、微波加热提取时间、微波功率、pH值和乙醇浓度等对提取率的影响。结果表明,牡丹雄蕊总生物碱的最佳提取工艺条件为:料液比1∶20,浸润时间24 h,微波加热提取时间15 min,微波功率500 W,pH值3和乙醇浓度80%。在此最佳工艺条件下,牡丹雄蕊中总生物碱提取率为2.16%。

Total alkaloids were extracted from peony stamens through microwave-assisted technique.Effects of the ratio of sample weight to solvent volume,soakage time,microwave radiation time,microwave power,pH value and ethanol concentration were studied. Result shows that the optimum technology of the total alkaloids extraction： 1 ∶ 20 of solid to liquid,24 h of soakage in solvent,15 min of microwave-assisted extraction,500 W of microwave power,3 of pH value and 80% ethanol. Under the optimized conditions,the extraction percent of total alkaloids from peony stamens is about 2. 16%.