云南人群系统性红斑狼疮与TNF微卫星基因座关系的研究

The Relation Between TNF Microsatellite Loci Polymorphism and Systemic Lupus Erythematosus in Yunnan People

位于MHC内的肿瘤坏死因子 (tomornecrosisfactor,TNF)基因区域有 5个微卫星基因座a、b、c、d和e,本研究调查了中国云南汉族系统性红斑狼疮 (systemiclupuserythematosus,SLE)与这几个微卫星基因座多态性的关系。本文用荧光标记引物和半自动基因扫描方法对云南地区 97例SLE患者及 79例健康对照者的这 5个微卫星基因座进行了基因分型 ,结果发现 ,SLE患者的TNFa1(P =0 0 2 0 6 ) ,c2 (P =0 0 0 0 0 )等位基因频率较正常人显著增高 ,而TNFa2 (P =0 0 16 3) ,c3(P =0 0 0 6 5 ) ,c4(P =0 0 0 12 ) ,d6 (P =0 0 44 8)等位基因频率则是正常对照较SLE患者高。同时我们还发现在这 5个微卫星基因座中 ,与白种人比较中国人出现了尚未发现的新等位基因。

We have investigated TNF microsatellite polymorphism in SLE A total of 97 Chinese Han SLE patients and 79 matched Chinese Han controls were studied in this study, TNF microsatellites a,b,c,d and e were typed using fluorescent labeled automated genescanning and genotyping TNFa1(P =0 0206), c2 (P =0 0000) allele frequencies were significantly increased in the SLE group comparing with controls,and TNF a2 (P=0 0163) c3(P=0 0065),c4(P=0 0012),d6(P =0 0448) allele frequencies were significantly increased in controls Meanwhile,we found some new alleles in Chinese which are different form those in Caucasoids.