补气活血方对慢性支气管炎肺气虚证大鼠肺组织细胞凋亡的影响

Effect of Buqi huoxue Decoction on Apoptosis in Chronic Bronchitis Rats with Deficiency of Lung-qi

目的探讨补气活血方对慢性支气管炎肺气虚证模型大鼠肺组织细胞凋亡的影响。方法 50只SD大鼠随机选取10只作为空白对照组,其余采用改良烟熏法复制慢性支气管炎肺气虚证大鼠模型,造模后按随机数字表法分为模型组、补气活血方高剂量组、补气活血方低剂量组、补肺汤组,每组10只。空白对照组和模型组灌服生理盐水,其余3组分别应用高、低剂量补气活血方和补肺汤进行灌胃干预。采用TUNEL法和流式细胞仪观察肺组织细胞凋亡情况。结果模型组大鼠肺组织炎性细胞凋亡率低于空白对照组,肺实质细胞凋亡率高于空白对照组(P<0.05);补气活血方高、低剂量组和补肺汤组肺组织炎性细胞凋亡率高于模型组,且补气活血方高剂量组高于低剂量组和补肺汤组(P<0.01);补气活血方高、低剂量组和补肺汤组肺组织实质细胞凋亡率低于模型组,且补气活血方高剂量组和低剂量组均低于补肺汤组(P<0.01,P<0.05)。结论补气活血方能够改善慢性支气管炎肺气虚证大鼠肺组织的形态,控制炎症及加强肺组织的自我修复,其机制与诱导肺组织内炎性细胞的凋亡,并抑制肺组织实质细胞的凋亡有关。

Objective To explore the effect of Buqi huoxue decoction on apoptosis of chronic bronchitis rats with deficiency of lung-qi. Methods Ten rats were randomly selected from 50 SD rats as control group,and models of the deficiency of lung-qi of chronic bronchitis（ CB） were established using the improved smoking-fumigated method in the other40 rats. The successful model rats were randomly divided into model group,high-dose group of Buqi huoxue decoction,low-dose group of Buqi huoxue decoction and tonifying lung recipe group（ n = 10 for each group） by random digits table.The control and model groups underwent normal saline irrigation,while the other three groups were treated with high and low doses of Buqi huoxue decoction and tonifying lung recipe. Apoptosis conditions were observed with TUNEL kit and flow cytometry. Results The percentage of inflammatory cells apoptosis in model group was significantly lower compared with that in control group（ P 〈 0. 05）,and the percentage of lung parenchyma cell apoptosis was significantly higher compared with that in control group（ P〈 0. 05）; the percentages of inflammatory cells apoptosis in high-dose,low-dose and tonifying lung recipe groups were significantly higher compared with that in model group,and the percentage in high-dose group was significantly higher compared with those in low dose and tonifying lung recipe groups（ P〈 0. 01）; the percentages of lung parenchyma cell apoptosis in high-dose,low-dose and tonifying lung recipe groups were significantly lower compared with that in model group,and the percentages in high-dose and low-dose groups were significantly lower compared with that in tonifying lung recipe group（ P 〈 0. 01,P 〈 0. 05）. Conclusion The Buqi huoxue decoction can improve deficiency of lung qi of pulmonary tissue morphology in chronic bronchitis rats. The mechanisms of controlling inflammation and strengthening the lung tissue self-repair may be related to the apoptosis induction of inflammatory cells in lung tissue and inhibition of lung parenchyma cell apoptosis.