高效液相色谱法测定栀姜口服液中栀子苷的含量

Content Detection of Geniposide in Zhijiang Oral Liquid by High Performance Liquid Chromatography

目的测定栀姜口服液中栀子苷的含量。方法应用高效液相色谱法（HPLC）,色谱柱：Hypersil ODS 2柱（4.6 mm×250 mm,5μm）;流动相：甲醇∶水（25∶75）;流速为1.0 ml/min;检测波长为238 nm;柱温为30℃;进样量为10μl。结果栀子苷在1.048～52.40μg/ml范围内与其峰面积线性关系良好（r=0.9 999）,平均回收率为99.49%,相对标准偏差为1.60%。结论 HPLC用于栀姜口服液中栀子苷的含量测定操作简便、准确可靠,有助于该制剂的质量控制和评价。

Objective To detect Geniposide content in Zhijiang oral liquid. Methods The chromatographic column was Hypersil ODS 2 column（ 4. 6 mm × 250 mm,5 μm）; the mobile phase consisted of methanol ∶ water（ 25∶ 75）; the flow rate was 1. 0 ml/min; the detection wavelength was at 238 nm; the column temperature was at30 ℃; the injection volume was 10 μl. Results The values of Geniposide within the range of 1. 048- 52. 40 μg /ml had good linear correlation with the values of peak area（ r = 0. 9 999）,the average recovery rate was 99. 49% with 1. 60% of relative standard deviation（ RSD）. Conclusion The high performance liquid chromatography（ HPLC） method is simple and accurate,and can be used for the quality control and evaluation of Geniposide in Zhijiang oral liquid.