四神丸治疗腹泻的实验研究

Experimental study on treatment of diarrhea by Sishen Pill

目的通过观察腹泻小鼠胃肠运动和腹泻大鼠结肠中胃肠肽蛋白表达的变化,探讨四神丸对腹泻的影响。方法采用大黄泻下法复制模型,以稀便级、腹泻率、稀便率、腹泻指数、碳末推进法观测小肠碳末推进率代表胃肠运动情况,将造模成功实验大鼠分为空白对照组、模型对照组、西药对照组、四神丸组,给药后对大鼠结肠通过免疫组织化学方法检测5-羟色胺(5-HT)、P物质(SP)蛋白表达的情况,观察四神丸对其干预作用。结果经大黄泻下法复制模型后,生大黄使小鼠的胃肠运动加快,大鼠结肠中5-HT和SP蛋白表达明显升高。四神丸可以减慢腹泻小鼠胃肠运动,降低腹泻大鼠结肠中5-HT和SP蛋白表达。结论四神丸有止泻作用,可明显抑制胃肠运动,可能是通过干预5-HT、SP蛋白表达而起作用。

Objective By animal experiment observed changes of gastrointestinal motility in mice with experimental diarrhea and the expression of gastrointestinal peptides protein in rat with experimental diarrhea, to investigate the effect of Sishen Pill on diarrhea. Methods Establish model by rhubarb purge, degree of diarrhea, the diarrhea rate, loose stools rate, diarrhea index, carbon particle propelling detection to observe the propellent rate of small intestine, represent gastrointestinal motility . Experimental rat of successful modeling were divided into four groups, blank control group, model group, western medicine control group, Sishen Pill group. After the last administration, the expression of 5-HT and the expression of SP were detected by immunohistochemical method in colon, to observe intervention effect of Sishen Pill. Results By establish model by rhubarb purge, rhubarb lead to accelerate gastrointestinal motility, the expression of 5-HT the expression of SP were enhanced in colon of rat. Sishen Pill could slow gastrointestinal motility in mice, the expression of 5-HT the expression of SP were reduced. Conclusions Sishen Pill could antidiarrheal, inhibit significantly gastrointestinal motility, intervene possibly the expression of 5-HT the expression of SP and play a role.