摘要
When monosomically introduced into wheat, the so called gametocidal chromosomes of wild species belonging to genus Aegilops , which is related to the genus Triticum , are known to induce chromosomal mutations, either lethal or sublethal, in gametes lacking the Aegilops chromosome. The chromosome mutation involves breakage in chromosomes or chromatids and repair or fusion of the broken ends, resulting in the generation of deletions and translocations. Two such chromosomes, chromosome 2C derived from Ae. cylindrica and chromosome 3C from Ae. triuncialis, have been used to induce chromosomal structural changes in each of the barley and rye chromosomes added to common wheat. In the progeny of the wheat lines that are disomic for the barley or rye chromosome and monosomic for the Aegilops chromosome, chromosome mutations involving the barley or rye chromosome occurred in more than 10% of the plants examined. Sequential chromosome banding (N banding or C banding) and in situ hybridization (FISH and/or GISH) revealed that there were many wheat alien translocations, as well as terminal deletions of the alien chromosomes. All these aberrant barley and rye chromosomes in common wheat would be useful for physical mapping of the respective alien chromosomes using DNA markers. Besides, alien to wheat translocations could be employed in wheat breeding programs. Terminal segments of the satellite of rye chromosome 1R have been transferred to the tips of different wheat chromosomes by the gametocidal action of chromosome 3C. By using this strategy, it is possible to obtain a 1R satellite segment carrying the disease resistance genes but not the Sec 1 locus, which is proximally located.
When monosomically introduced into wheat, the so called gametocidal chromosomes of wild species belonging to genus Aegilops , which is related to the genus Triticum , are known to induce chromosomal mutations, either lethal or sublethal, in gametes lacking the Aegilops chromosome. The chromosome mutation involves breakage in chromosomes or chromatids and repair or fusion of the broken ends, resulting in the generation of deletions and translocations. Two such chromosomes, chromosome 2C derived from Ae. cylindrica and chromosome 3C from Ae. triuncialis, have been used to induce chromosomal structural changes in each of the barley and rye chromosomes added to common wheat. In the progeny of the wheat lines that are disomic for the barley or rye chromosome and monosomic for the Aegilops chromosome, chromosome mutations involving the barley or rye chromosome occurred in more than 10% of the plants examined. Sequential chromosome banding (N banding or C banding) and in situ hybridization (FISH and/or GISH) revealed that there were many wheat alien translocations, as well as terminal deletions of the alien chromosomes. All these aberrant barley and rye chromosomes in common wheat would be useful for physical mapping of the respective alien chromosomes using DNA markers. Besides, alien to wheat translocations could be employed in wheat breeding programs. Terminal segments of the satellite of rye chromosome 1R have been transferred to the tips of different wheat chromosomes by the gametocidal action of chromosome 3C. By using this strategy, it is possible to obtain a 1R satellite segment carrying the disease resistance genes but not the Sec 1 locus, which is proximally located.
出处
《遗传》
CAS
CSCD
北大核心
2001年第1期49-49,共1页
Hereditas（Beijing)
