兖州卷柏的肽类化学成分及对低氧/复氧诱导的PC-12细胞损伤的保护作用

Peptide Chemical Constituents from Selaginella involven Spring and Their Protective Effects on Anoxic /Reoxygenation-Induced PC-12 Cell Damage

采用聚酰胺、硅胶柱层析、Sephadex LH-20和制备液相等分离方法对卷柏属植物兖州卷柏的化学成分进行分离纯化,根据理化性质和现代波谱技术鉴定了11个化合物：aurantiamide（1）、aurantiamide acetate（2）、Nbenzoyl-L-phenylalaninol（3）、neoechinulin A（4）、5,7-二羟基色原酮（5）、3-（3-羟基-苯基）-丙酸（6）、3-（3-羟基-苯基）-丙酸甲酯（7）、肉桂酸（8）、（1H-indol-3-yl）oxoacetamide（9）、3-甲醛-1H-吲哚（10）、3-甲酸-1H-吲哚（11）,以上11个化合物首次从该植物中分离得到,且为首次从卷柏属植物中得到。采用Hoechst Staining法评价化合物1~4四个肽在低氧/复氧诱导的PC-12细胞损伤中的保护作用,结果表明4个肽类化合物在低氧复氧诱导的PC-12中表现出较强的抗氧化作用,并具有剂量依赖性,对低氧/复氧诱导的PC-12细胞损伤呈现出一定的保护作用。

Eleven compounds were isolated and purified from Selaginella involven Spring by polyamide,macmpomus resin and Sephadex LH-20 eohmm chromatography, and their structures were elucidated as anrantiamide （ 1 ）, anrantiamide acetate （2）, N-benzoyl-L-phenylalaninol （ 3 ）, neoechinulin A （4）, 5,7-dihydroxy Chromone （ 5 ）, 3 - （ 3 -hydroxy-phenyl） propionic acid （6）,3- （3 -hydroxy-phenyl ） methyl pmpionate （7）, cinnamic acid （8）, （ 1H-indol-3 -yl ） oxoacetamide （9）, 1H-Indole-3 -carboxaldehyde （10）, 1H-indole-3 -carboxylieacid （11） by chemical and modem spectroscopic methods. The eleven compounds were isolated from genus SelagineUa for the first time. The protective effects of compounds 1- 4 on anoxie/reoxygenation-induced PC-12 cell damage were evaluated by Hoechst Staining method. The four compounds showed strong antioxidant effect on the PC-12 cell damage induced by anoxic/reoxygenation,and had a dose dependent effect. Compounds 1-4 showed moderate protective effects on the PC-12 cell damage induced by anoxic/reoxygenation.