摘要
目的调查215株湖州地区临床分离铜绿假单胞菌对氨基糖苷类抗生素的耐药性和16S rRNA甲基化酶基因分布情况。方法收集2011年1月至2012年12月湖州地区临床分离铜绿假单胞菌215株,琼脂稀释法测定5种氨基糖苷类抗菌药物(庆大霉素、阿米卡星、妥布霉素、伊帕米星、奈替米星)的MIC值;PCR检测armA、rmtA、rmtB、rmtC、rmtD和npmA六种氨基糖苷类16S rRN甲基化酶基因,序列分析明确基因型。测定产16S rRNA甲基化酶菌株对常见抗菌的敏感性,并检测碳青霉烯耐药株产碳青霉烯酶情况。结果铜绿假单胞菌对异帕米星敏感率最高为81.4%,对5种氨基糖苷类抗生素全部耐药的22株菌株中,17株检出armA基因;未发现其他16S rRNA甲基化酶基因阳性菌株。17株armA阳性菌株对碳青霉烯类抗生素耐药5株(耐药率为29.4%),对头孢他啶、头孢吡肟、哌拉西林/他唑巴坦、环丙沙星耐药率均超过40%。5株碳青霉烯耐药菌株中检测到2株产VIM-2型金属碳青霉烯酶。结论铜绿假单胞菌对氨基糖苷类抗生素耐药率高,检测到16S rRNA甲基化酶基因armA。产16S rRNA甲基化酶铜绿假单胞菌耐药性强,部分菌株同时产金属碳青霉烯酶,给临床抗感染治疗及院内感染控制带来挑战。
Objective To investigate the resistance to aminoglycosides and prevalence of 16S rRNA methylases genes in Pseudomonas cteruginosa isolates isolated from a hospital in Huzhou. Methods Total of 215 isolates of Pseudornonas aeruginosa were collected from a hospitals of Huzhou, through January, 2011 to December, 2012. The minimum inhibitory concentrations （MIC） of these strains were examined by agar dilution method. Six 16S rRNA methylase genes, arrnA, rmtA, rmtB, rmtC, rmtD and nprnA, were detected by PCR. PCR products were purified and sequenced. Susceptibility of 16S rRNA methylases-producing isolates were detected, and carbapenem coding genes were detected among carbapenem-resistant strains. Results The highest susceptibility rate among the 5 antimicrobials was isepamicin, 81.4%. 17 armA-positive isolates were identified from 22 Pseudomonas aeruginosa isolates which were high-level resistance to five aminoglycosides. No other 16S rRNA methylase gene was detected. Five of the 17 annA-producing strains were resistant to earbapenem （ 29.4% ）. Resistance of ceftazidime, cefepime, piperacillin/tazobactam, ciprofloxacin was all above 40%. VIM-2 gene was detect in 2 of the 5 carhapenem-resistant strains. Conclusion The resistant rate to aminoglycosides is high in Pseudomonas aeruginosa. ArmA-producing Pseudomonas aeruginosa isolates are detected in Zhejiang province.
出处
《中国微生态学杂志》
CAS
CSCD
2014年第6期663-666,共4页
Chinese Journal of Microecology
基金
卫生部临床重点专科建设项目
