Valen席夫碱单核铜配合物的合成、热化学性质及其对粟酒裂殖酵母细胞作用的热动力学研究

Synthesis, thermochemical and thermokinetic studies on the mononuclear copper Valen Schiff base complex

以甲醇为溶剂,邻香草醛缩乙二胺席夫碱(H2vanen)与一水合醋酸铜[Cu(OAc)2·H2O]反应,合成了一种Valen席夫碱单核铜配合物[Cu(vanen)(H2O)].以盖斯定律为理论基础,设计了一个合理可行的热化学循环.根据该热化学循环,依次测定了各样品的标准摩尔溶解焓,进而求得配位反应的标准摩尔反应焓(θr m?H)为?(16.61±0.54)kJ/mol.再结合其他相关的文献值,求得配合物的标准摩尔生成焓(θf m?H)为?(756.5±2.4)kJ/mol.在32℃条件下,用热导式等温微量热仪测定了不同浓度配合物作用下粟酒裂殖酵母细胞的产热曲线,探讨了不同浓度的配合物对酵母菌生长的影响,分析了配合物的浓度与酵母菌代谢的生长速率常数k、抑制率I、最大发热功率Pmax、半抑制浓度IC50以及达到最大发热功率所需时间tmax等动力学参数之间的关系.分析结果表明,配合物对粟酒裂殖酵母细胞的生长具有低浓度促进、高浓度抑制的Hormesis效应.

A mononuclear salen-type complex [Cu（vanen）（H2O）] was synthesized from the ligand [H2vanen = N,N＇- ethylene-bis（3-methoxysalicylideneimine）] and Cu（OAc）2·H2O. Based on Hess＇ law, a reasonable thermochemical cycle was designed. According to the cycle, the standard molar enthalpies of dissolution of samples in the calorimetric solvent of DMF （N,N-dimethylformamide） were determined, respectively. The standard molar enthalpy change of the coordination reaction （ΔrHmθ） was calculated to be -（16.61 ± 0.54） kJ/mol. Combined with some relevant literature data, the standard molar enthalpy of formation （ΔfHmθ） of the complex was obtained to be -（756.5 ± 2.4） kJ/mol. At the temperature of 32 ℃, the metabolic thermogenetic curves of S. pombe under the influence of the complex with different concentration were determined by using a TAM air isothermal calorimeter. The relationships of the concentration of the complex with the microbial growth rate constant （k） of S. pombe, inhibition ratio （I）, the half inhibition concentration （IC50）, the maximum heat output power （Pmax） and the time it took （tmax） were analyzed. The results indicated that the complex possessed the Hormesis effect, which promoted the growth of S. pombe at lower concentration, but inhibited the growth at higher concentration.