摘要
目的研究中药单体蛇床子素(Osthole,Ost)与5-FU(Fluorouracil,5-FU)在人胃癌细胞株BGC-823以及人大肠癌细胞株LOVO中的体外相互作用。方法采用MTT法检测单药及联合用药对两株肿瘤细胞株的作用;利用中效原理评价两药之间的相互作用;利用流式细胞仪检测单药及联合用药对肿瘤细胞凋亡和周期的影响。结果Ost和5-FU对两株肿瘤细胞株均具有增殖抑制作用;作用于BGC-823细胞时,当Fa<0.65时,CI<1,两药合用效应为协同作用;作用于LOVO细胞时,当0.05<Fa<0.90时,CI<1,两药合用效应为协同作用。结论 Ost联合5-FU作用于BGC-823细胞在低剂量合用时产生协同效应,协同机制可能与诱导细胞凋亡及G2/M期阻滞相关。
Objective To study the in vitro interactions between osthole （ost） and fluorouracil （5-FU） on human gastric carcinoma cell lines BGC-823 and the human colon cancer cell lines LOVO. Method For single agent and combined medication, the effects on two tumor cell lines, the interactions, and the influences on tumor cell cycle and apoptosis were analyzed using MTT method, median-effect principle and flow cytometry, respectively. Result The combination of ost and 5-FU inhibited the proliferation of the two tumor cell lines; For BGC-823 cells, when Fa〈0. 65, CI〈 1, the combined effects of the two medicine were synergies; For LOVO cells, when 0. 05〈Fa〈0. 90, CI〈1, the effects were also synergies. Conclusion The combined effects of ost and 5-FU on BGC-823 cells were synergistic when in low doses, and the mechanism may be related to apoptosis induced and the block of G2/M phase.
出处
《癌症进展》
2014年第3期278-284,共7页
Oncology Progress
基金
南京市医学科技发展项目(ZKX09017)
