离子交联法制备聚唾液酸/壳聚糖纳米粒

Preparation of polysialic acid /chitosan nanoparticles via ionic gelation

以聚唾液酸和壳聚糖为原料,多聚磷酸钠为交联剂,采用离子交联法制备聚唾液酸/壳聚糖纳米粒。结果表明:当聚唾液酸质量浓度为1.25 mg/mL,壳聚糖质量浓度为1.00 mg/mL,壳聚糖与聚唾液酸质量比为2∶1,多聚磷酸钠质量浓度为0.15 mg/mL,滴加速度为1滴/s时,所制备的聚唾液酸/壳聚糖纳米粒粒径最小,平均粒径为217.2 nm,纳米粒粒径分散指数为0.236。以牛血清白蛋白(BSA)为模型药物,包封率为(44.95±1.22)%,载BSA率为(28.90±0.78)%。离子交联法制备聚唾液酸/壳聚糖纳米粒操作简单快捷,不使用有机溶剂,所得纳米粒粒径较小,有望成为蛋白类药物的载体。

Polysialic acid/chitosan nanoparticles are developed via ionic gelation of two polysaccharide-based molecules,negatively charged polysialic acid and positively charged chitosan.Sodium tripolyphosphate is added as crosslinking agent.The minimum nanoparticle size can be achieved under the following conditions：1.25 mg/mL of polysialic acid,1.00 mg/mL of chitosan,2：1 mass ratio of chitosan to polysialic acid,0.15 mg/mL of tripolyphosphate and 1 drop/s of drop acceleration.The average size of the nanoparticle is 217.2 nm with a particle size distribution of 0.236.Using bovine serum albumin as a model drug,the loading efficiency and loading capacity are 44.95％ and 28.90％,respectively.Preparation of polysialic acid/chitosan nanoparticles via ionic gelation is simple,quick and free of organic solvent.Such kind of small size polysialic acid/chitosan nanoparticle is a potential vehicle for the delivery of drug proteins.