分子印迹固相萃取测定水中苯并三唑类衍生物

Determination of benzotriazoles in water by molecularly imprinted solid-phase extraction

建立了分子印迹固相萃取高效液相色谱法分析地表水中苯并三唑类衍生物的方法.环境水样经0.22 μm滤膜过滤后用盐酸（1∶1）调至pH为3,100 mL过分子印迹固相萃取柱,用4 mL3％异丙醇的二氯甲烷溶液淋洗,4 mL甲醇洗脱.结果表明,4种目标化合物在0.06 ～ 30 μg/mL范围内均呈良好的线性关系,线性相关系数均＞0.99,仪器检出限为0.9～2.2 μg/L.空白自来水样加标回收率为75.8％ ～92.9％,相对标准偏差（RSD,n=3）为2.3％ ～8.6％.

A simple method based on molecularly imprinted solid-phase extraction （MISPE） coupled with high performance liquid chromatography （HPLC） is developed for determination of benzotriazoles in surface water.The optimum extraction process is as follows：3 of pH for the sample,100 mL of the sampling volume,4 mL of 3％ （V/V）isopropanol in dichloromethane as washing solvent and 4 mL of methanol as eluting solvent.The results show that good linear relationship can be observed in the ranges of 0.06-30 μg/mL with correlation coefficients over 0.99.The instrumental limits of detection for the target compounds are in the range of 0.9-2.2 μg/L.The average recoveries of target compounds are 75.8％-92.9％ and the corresponding relative standard deviations （RSD,n =3） are 2.3％-8.6％.