乳腺癌实施化疗后癌干细胞同P-糖蛋白及耐药蛋白在残存癌组织中表达的相关性

Breast cancer stem cells after chemotherapy with P- glycoprotein and resistant protein expression of correlation in the residual carcinoma tissue

目的研究乳腺癌实施化疗后癌干细胞、P-糖蛋白及耐药蛋白在残存癌组织中的表达情况。方法将本院2012年5月至2013年5月收治的53例乳腺癌患者作为研究对象,比较化疗前后癌组织中癌干细胞、P-糖蛋白及耐药蛋白表达情况。结果患者化疗后,残留癌细胞中的癌干细胞含量上升且形成的微球体直径增加,与化疗前相比差异具有显著性(t=6.3615,191.3086;P=0.0000);患者化疗后,残留癌细胞中癌干细胞内谷胱甘肽转移酶π、拓补异构酶Ⅱ的阳性表达率上升,与化疗前相比差异具有显著性(χ2=3.9775,4.4002;P=0.0461,0.0359);患者化疗后,残留癌组织中P-糖蛋白表达水平上升,而耐药蛋白表达水平上升极其明显;与化疗前相比差异极其显著(t=6.5045,11.2765;P=0.0000)。结论乳腺癌患者在化疗后,残存癌组织中的癌干细胞含量上升、P-糖蛋白及耐药蛋白的阳性表达率上升,产生一定的耐药性。

ObjectiveTo study the breast cancer stem cells after chemotherapy, P-glycoprotein and resistant protein expression in the residual carcinoma tissue.MethodsFrom May 2012 to May 2013 were 53 cases of breast cancer patients as the research object, compared before and after chemotherapy cancer stem cells in cancer tissue, P-glycoprotein and resistant protein expression.ResultsThe patients after chemotherapy, residual carcinoma cells in cancer stem cells content increased and the formation of the microsphere diameter increase, signiifcant difference compared with before treatment （t=6.3615, 191.3086;P=6.3615）. Cancer stem cells in patients after chemotherapy, residual cancer cells within the glutathione transferase PI, the topology isomerase II the positive expression rate of rise, compared with before treatment with statistical signiifcance （χ2=3.9775, 3.9775;P=0.0461, 0.0359）; after chemotherapy, patients with residual P-glycoprotein expression level in cancerous tissue, while the rise in drug-resistant protein expression and its obvious; significant difference compared with before treatment （t=6.5045, 11.2765;P=6.5045）.ConclusionIn patients with breast cancer after chemotherapy, residual carcinoma cancer stem cells in tissue levels rise, and P-glycoprotein and resistant protein positive expression rate of rise, produce certain resistance.