肺移植后闭塞性细支气管炎大鼠模型建立及其miRNA表达谱分析

Establishment of a Standard Obliterative Bronchiolitis Rat Model for Research on MicroRNA Expression Profiles

目的 探索建立大鼠原位气管移植模拟肺移植后闭塞性细支气管炎（obliterative bronchiolitis,OB）的病理生理过程,为研究肺移植后OB中microRNA （miRNA）表达谱分析提供理想的动物模型.方法 通过近交系大鼠原位气管移植,建立模拟肺移植后发生OB的动物模型.模型成功后使用HE染色对气管移植模型进行评估;提取移植气管组织进行microarray芯片检测,同时应用实时定量RT-PCR（RT-qPCR）验证miRNAs芯片结果的可靠性.结果 成功建立了大鼠原位气管移植的模型26/30例（成功率为86.67％）;术后4周移植气管病理检查提示“移植气管炎症细胞浸润和纤维组织增生”,模型OB的发生率为100％.miRNA芯片筛选出移植气管OB组织中表达上调15个和下调14个;验证其中显著上调miR-146a、miR-155和显著下调miR-451结果与检测结果具有较好的一致性.结论 原位气管移植能够稳定形成模拟肺移植后OB的气道阻塞性病变,可适用于肺移植后OB的miRNA表达谱分析研究的动物模型.

Objective Exploration on simulation establishment of rat Orthotopic tracheal transplantation obliterative bronchiolitis （OB） after lung transplantation,pathophysiological process,for the study of lung transplantation in OB microRNA （miRNA） expression profiles provide an ideal animal model.Methods By Orthotopic tracheal transplantation in inbred rats,establish simulation of OB after lung transplantation animal models according to the designed groups （n=6 pairs）.After successful models HE used dye to assess the tracheal transplantation model; The total RNA was extracted from transplanted trachea tissues by Trizol.Small miRNA was isolated from total RNA,hybridized on miRNA microarray,and the microarray results confirmed by quantitative real-time reverse transcription-polymerase chain reaction（RT-qPCR）.Results The survival rate of established model was 86.67％ （26/30） beyond four weeks.The histology suggested that the trachea endothelial cells in experiment group were damaged,accompanied by enhanced inflammatory cell infiltration and fibroproliferation.Incidence of obliterative bronchiolitis in mice of experimental group was approximately 1 00 ％.Compared with the normal control group,there were 29 specific miRNAs in the model control group with the expression difference of more than 2 times by 15 up-regulation miRNAs and 14 down-regulating miRNAs,verify which significantly increase miR-146a,miR-155,and significantly reduced miR-451 and test results with good consistency.Conclusion Stability simulation of Orthotopic tracheal transplantation OB obstructive airway disease after lung transplantation can be applied after lung transplantation animal models of miRNA expression profiles in OB research.