安神定志灵对注意力缺陷多动障碍模型大鼠自发活动、焦虑及空间学习能力的影响

Effects of Anshen Dingzhi Ling on Spontaneous Hyperactivity,Anxiety and Space Learning and Memory in Attention Deficit Hyperactivity Disorder Rats

目的 探讨中药复方安神定志灵对注意力缺陷多动障碍（ADHD）模型大鼠自主活动、焦虑及空间学习能力的影响.方法 将SHR大鼠随机分为模型组、阳性对照托莫西汀组、安神定志灵低中高剂量组,每组8只,另将8只WKY大鼠设为正常组,灌胃4周.分别在给药前,给药3周后行旷场实验、高架十字迷宫及水迷宫.结果 旷场试验中安神定志灵低剂量组（P＜0.05）及托莫西汀组（P＜0.01）可减少大鼠总活动路程,Morris水迷宫实验中均可增加穿越平台次数（P＜0.01）,同时安神定志灵组可减少大鼠进入开臂次数及停留时间（P＜0.01）.结论 安神定志灵可减少大鼠自发活动,缓解焦虑行为,改善大鼠空间记忆学习能力.

Objective To explore the effects on brain monoamine neurotransmitter levels and potential action of mechanism of Anshen Dingzhi Ling（ADL） in the treatment of attention deficit hyperactivity disorder （ADHD） rats.Methods Forty SHR rats were randomly divided into five groups：ADHD model group,Atomoxetine groups,ADL-low-dose groups,ADL-medium-dose groups,and ADL-high-dose groups,at the same time eight WKY rats were set as the normal control groups.All the rats were gavaged for four weeks in a row.The open field,elevated plus maze （EPM）,Morris water maze were employed to observe the changes in the animal behavior before intragastric administration and 3 weeks after the administration.Results In open field test,ADL low-dose group （P＜0.05） and Atomoxetine group （P＜0.01） reduce the total distance of rats,and both of groups can increase the number of cross platform （P＜0.01） in Morris water maze test.At the same time ADL can reduce the frequency and Residence time that rats into the open arms （P＜0.01） in elevated plus maze.Conclusion ADL decreased the spontaneous hyperactivity,relieve anxiety behavior and improve the space learning and memory.