期刊文献+

核仁素蛋白调节嗅觉介导素4mRNA表达的研究

Olfaetomedin4 mRNA expression regulated by nucleolin protein
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摘要 目的:探讨核仁素(nucleolin,NCL或C23)蛋白与嗅觉介导素4(olfaetomedin 4,OLFM4)mRNA之间是否存在相互作用,以及NCL对OLFM4转录水平的调控。方法:本研究利用RNA染色质免疫共沉淀(RNA chromatin immunoprecipitation assay,RNA chip)结合RT-PCR确定NCL是否能与OLFM4 mRNA相互作用,以及双荧光素酶报告基因试验检测NCL对OLFM4报告基因的影响。结果:在SGC-7901细胞内,NCL能与OLFM4 mRNA结合,且过表达NCL质粒的相对荧光素酶活性约为对照质粒的1.15倍,敲低NCL质粒的相对荧光素酶活性约为对照质粒的0.8倍,NCL对OLFM4具正向调节作用。结论:NCL与OLFM4mRNA可以相互结合,且NCL可以通过增加OLFM4转录水平和延长OLFM4 mRNA的半衰期来增加OLFM4 mRNA的稳定性。 Objective:To explore the interaction between olfaetomedin 4(OLFM4)mRNA and nucleolin protein and to discuss the regulatory effect of nucleolin protein on the transcription level of OLFM4. Methods:RNA chromatin chromatin immunoprecipitation assay(RNA chip)combined with RT-PCR was performed to identify whether OLFM4 interacted with nucleolin and the effects of nucleolin on the OLFM4 were detected by dual-luciferase reporter assay. Results:OLFM4 directly interacted with the nucleolin in SGC-7901 cells. Relative luciferase activity of over expression of nucleolin plasmid was about 1.15 times of the control plasmid;relative luciferase activity of knockdown of nucleolin plasmid was about 0.8 times of the control plasmid;nucleolin had positive moderating effect on OLFM4. Conclusions:OLFM4 directly interacts with the nucleolin and nucleolin stabilizes the OLFM4 mRNA by increasing OLFM4 transcription and extending OLFM4 mRNA half-life.
出处 《重庆医科大学学报》 CAS CSCD 北大核心 2014年第5期657-660,共4页 Journal of Chongqing Medical University
基金 国家自然科学基金资助项目(编号:81001017)
关键词 核仁素 嗅觉介导素4 RNA染色质免疫共沉淀(RNA Chip) 双荧光素酶报告基因试验 nucleolin olfaetomedin4 RNA chromatin immunoprecipitation assay(RNA chip) dual-luciferase reporter assay
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参考文献15

  • 1Aung PP,Oue N,Mitani Y,et al.Systematic search for gastric cancer-specific genes based on SAGE data:melanoma inhibitory activity and matrix metalloproteinase-10 are novel prognostic factors in patients with gastric cancer[J].Oncogene, 2006,25(17) :2546-2557.
  • 2Greco A,Arata L,Soler E,et al.Nucleolin interacts with USI1 protein of herpes simplex virus 1 and is involved in its trafficking[J].J Vi- rol,2012,86(3) : 1449-1457.
  • 3Soundararajan S, Chen W, Spicer EK, et a/.The nucleolin targeting aptamer AS1411 destabilizes Bcl-2 messenger RNA in human breast cancer cells[J].Cancer Rcs,2008,68(7) : 2358-2365.
  • 4Zhang J ,Tsaprailis G, Bowden GT.Nucleolin stabilizes Bcl-XL messenger RNA in response to UVA irradiation[J].Cancer Res, 2008,68 (4) : 1046-1054.
  • 5Jiang Y,Xu XS, Russell JE.A nucleolin-binding 3' untranslated region element stabilizes beta-globin mRNA in vivo[J].Mol Cell Biol, 2006,26(6) : 2419-2429.
  • 6Mongelard F,Bouvet P.AS-1411,a guanosine-rich oligonucleotide aptamer targeting nucleolin for the potential treatment of cancer,including acute myeloid leukemia[J].Curr Opin Mol Ther,2010,12 (1): 107-114.
  • 7Farin K,Di Segni A, Mor A,et al.Structure-function analysis of nu- cleolin and ErbB receptors interactions[J].PLoS One,2009,4(7):e6128.
  • 8Tajrishi MM,Tuteja R,Tuteja N.Nucleolin:the most abundant muhifunctional phosphoprotein of nucleolus[J].Commun Integr Biol, 2011,4 (3) : 267-275.
  • 9Finger LD,Trantirek L,Johansson C,et al.Sloution structures of stem-loop RNAs that bind to the two N-terminal RNA-binding do- mains of nucleolin[J].Nucleic Acids Reserrch, 2003,31 (22) : 6461-6472.
  • 10Piccinelli P,Samuelsson T.Evolution of the iron-responsive element[J].RNA, 2007,13 (7) : 952-966.

二级参考文献36

  • 1熊高飞,熊向阳,张吉翔.mRNA结构及其稳定性的关系[J].细胞生物学杂志,2006,28(4):513-517. 被引量:7
  • 2胡云清.高等真核生物中mRNA稳定性的控制[J].国外医学(遗传学分册),1997,20(1):44-45. 被引量:1
  • 3尹昌林,吕胜青,黄轶,李光辉,唐莉,杨辉.pSiRNA-Notch1逆转录病毒载体的构建及对恶性脑胶质瘤细胞的作用[J].第三军医大学学报,2007,29(12):1211-1214. 被引量:4
  • 4Zhang X, Huang Q, Yang Z, et al. GW112, a novel antiapoptotic protein that promotes tumor growth [ J]. Cancer Res, 2004, 64 (7): 2474 - 2481.
  • 5Liu W, Chen L, Zhu J, et al. The glycoprotein hGC-1 binds to cadherin and lectins [J]. Exp Cell Res, 2006, 312(10) : 1785 -1797.
  • 6Oue N, Sentani K, Noguchi T, et al. Serum olfactomedin 4 (GW112, hGC-1 ) in combination with Reg IV is a highly sensitive biomarker for gastric cancer patients [ J]. Int J Cancer, 2009, 125 (10): 2383- 2392.
  • 7Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T) ) method [J]. Methods, 2001,25(4):402-408.
  • 8Taulli R, Accomero P, Follenzi A, et al. RNAi technology and lentiviral delivery as a powerful tool to suppress Tpr-Met-mediated tumorigenesis[J]. Cancer Gene Ther, 2005, 12(5) : 456 -463.
  • 9Brummelkamp T R, Bernards R, Agami R. Stable suppression of tumorigenicity by virus-mediated RNA interference [ J ]. Cancer Cell, 2002, 2 (3) : 243 - 247.
  • 10Yasui W, Oue N, Aung P P, et al. Molecular-pathological prognostic factors of gastric cancer: a review[J]. Gastric Cancer, 2005, 8(2) : 86 - 94.

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