细胞跨膜Notch配体4和血管内皮生长因子蛋白在子宫内膜异位症组织中的表达及意义

Expression and significance of delta-like ligand 4 and vascular endothelial growth factor in endometriosis

目的:探讨细胞跨膜Notch配体4(delta-like ligand 4,DLL4)蛋白和血管内皮生长因子(vascular endothelial growth factor,VEGF)在子宫内膜异位症(endometriosis,EMs)组织中的表达及意义。方法:采用免疫组织化学SP法检测48例EMs的异位内膜(A组)、37例在位内膜(B组)及30例正常子宫内膜组织(C组)中DLL4和VEGF蛋白的表达。结果:(1)A、B、C 3组中,DLL4蛋白的平均积分光密度(integral optical density,IOD)值分别为(145.01±10.47)、(115.56±10.87)、(90.08±8.56),VEGF的IOD值分别为(148.48±8.36)、(122.79±5.68)、(108.75±7.39),组间差异均有统计学意义(F=279.113和r=296.049,P均<0.05)。(2)A、B两组中DLL4和VEGF蛋白的表达存在正相关(r=0.828和r=0.773,P均<0.05),C组中二者的表达无相关性。(3)A、B两组中Ⅰ、Ⅱ期DLL4和VEGF蛋白的表达均显著低于Ⅲ、Ⅳ期(P<0.05)。结论:DLL4、VEGF蛋白可能在EMs的发生发展中发挥着重要作用。

Objective：To investigate the expression and significance of delta-like ligand 4（DLL4） and vascular endothelial growth factor（VEGF）in endometriosis（EMs）. Methods：Expressions of DLL4 and VEGF in 48 cases of ectopic endometrium（group A）,37 cases of eutopic endometrium（group B）and 30 cases of normal endometrium（group C）was detected by the immunohistochemical SP method. Results：（1）In groups A,B,C,expression of DLL4 was（145.01±10.47）,（115.56±10.87）and（90.08±8.56）while expression of VEGF was（148.48±8.36）,（122.79±5.68）and（108.75±7.39）,with significantly differences among 3 groups（F=279.113 and F=296.049,P＆lt;0.05）.（2）In group A and B,expressions of DLL4 and VEGF showed a positive correlation（r=0.828 and r=0.773, P＆lt;0.05）,but in group C,their expression showed no correlation.（3）In group A and B,expression of DLL4 and VEGF at stageⅠand Ⅱ were significantly lower than that at stage Ⅲ and Ⅳ（P＆lt;0.05）. Conclusions：DLL4 and VEGF may play an important role in the pathogenisis of EMs.