摘要
目的:利用聚焦超声(focused ultrasound,FU)热效应对兔眼角膜进行辐照,观察其生物效应的变化。方法:13只新西兰兔(26眼)根据辐照功率及辐照角膜直径的不同随机分为A、B、C组。设计A组辐照功率为1 W,辐照角膜直径为9 mm;B组辐照功率为1.5 W,辐照角膜直径为9 mm;C组辐照功率为1 W,辐照角膜直径为7 mm。分别于术前及术后3、10 d、1月、2、3月对术眼进行裂隙灯、自动验光仪、角膜地形图、内皮细胞计数仪及非接触眼压的检查及记录。结果:术后即刻24眼(92.3%)角膜辐照区上皮缺损,术后3 d 26眼(100%)角膜上皮完整。角膜中央曲率于术后1月开始降低,随观察时间延长呈逐渐降低趋势;屈光度术后呈正向增加趋势,向远视方向发展,与角膜中央曲率变化趋势一致。非接触眼压术后3、10 d降低,术后1、2、3月恢复到术前水平。角膜内皮细胞计数术前术后无明显变化。结论:辐照兔眼周边角膜引起角膜中央曲率降低。FU应用于兔眼角膜安全性较高。
Objective:To study the biological effects of focused ultrasound(FU)on rabbit’s cornea. Methods:Totally 26 eyes of 13 New Zealand rabbits were randomly divided into A,B,C groups according to FU power and corneal diameter. Group A was irradiated using power of 1 W and corneal diameter of 9 mm;group B was irradiated using power of 1.5 W and corneal diameter of 9 mm;group C was irradiated using power of 1 W and corneal diameter of 7 mm. Rabbits were examined with slit-lamp microscope,auto refractometer,corneal topography,specular microscopy and non-contact tonometer before the operation and on 3,10 d and at 1,2,3 months after the operation respectively. Results:Twenty-four eyes(92.3%)had corneal epithelium defect in irradiated area immediately after the operation and all 26 eyes(100%)got complete corneal epithelium on 3 d after the operation. Curvature of the central corneal was reduced gradually 1,2,3 months postoperatively(P<0.05);diopter was increased gradually(P<0.05),presenting a trend to hyperopia,the same as the change of central corneal curvature. Non-contact intraocular pressure was reduced on 3,10 d postoperatively(P<0.05)and was recovered at 1,2,3 months postoperatively. Corneal endothelial cell density was not statistically significant between preoperative and postoperative observation. Conclusions:FU irradiation at the peripheral cornea of rabbits can reduce central corneal curvature and is safe when applied in rabbit’s cornea.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2014年第5期701-705,共5页
Journal of Chongqing Medical University
基金
重庆市卫生局重点课题(编号:2010-1-25)
关键词
聚焦超声
兔
角膜
生物效应
focused ultrasound
rabbit
cornea
biological effects