载多西紫杉醇脂质微泡联合超声靶向微泡破裂对兔VX2舌癌颈部淋巴转移瘤作用的初步研究

Preliminary study of effect of docetaxel-loaded lipid microbubbles combined with ultrasound targeted microbubbles destruction on rabbit VX2 cervical lymph node metastatic carcinoma

目的:初步探讨载多西紫杉醇脂质微泡(docetaxel-loaded lipid microbubbles,DLLM)联合超声靶向微泡破裂(ultrasound targeted microbubbles destruction,UTMD)对兔VX2舌癌颈部淋巴转移瘤的治疗作用。方法:成功建立兔VX2舌癌颈部淋巴转移瘤动物模型30只,随机分为A组、B组、C组、D组、E组、F组(n=5):A组为单纯药物组、B组为单纯载药微泡组、C组为药物+超声组、D组为单纯微泡+超声组、E组为载药微泡+超声组、F组为对照组。治疗前后用超声测量各组动物颈部淋巴转移瘤大小,计算瘤体体积和抑瘤率;比较各组动物颈部淋巴转移瘤组织中CD34、微血管密度(microvessel density,MVD)及血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达。结果:E组实验动物颈部淋巴转移瘤的抑瘤率高于其他组(P<0.05),与其他各组相比差异有统计学意义;E组实验动物颈部淋巴转移瘤中CD34表达水平最低,MVD、VEGF表达水平显著低于其他各组(P<0.01)与其他各组相比差异有统计学意义。结论:DLLM联合UTMD不仅能在宏观表现上抑制兔VX2舌癌颈部淋巴转移瘤生长,更能一定程度上从分子生物学水平明显抑制兔VX2舌癌颈部淋巴转移瘤的转移、生长。

Objective：To study the treatment effect of docetaxel-loaded lipid microbubbles（DLLM）combined with ultrasound targeted microbubbles destruction（UTMD）on rabbit VX2 cervical lymph node metastatic carcinoma. Methods：Thirty rabbit VX2 cervical lymph node metastatic carcinoma model were successfully established and were randomly divided into 6 groups（A group,B group,C group,D group,E group,F group,n=5）. A group used docetaxel,B group used DLLM,C group used docetaxel ＋ UTMD,D group used lipid microbubbles ＋ UTMD,E group used DLLM ＋UTMD and F group was control group. Volume and inhibition rate of cervical lymph node metastatic carcinoma in each group before and after the treatment were detected and compared. Expression of CD34 and microvessel density（MVD）and vascular endothelial growth factor（VEGF）was compared among different groups. Results：After the treatment,inhibition rate was higher in E group than in other groups（P＆lt;0.05）and the expression of CD34 and MVD and VEGF was significantly lower in E group than in other groups（P＆lt;0.01）. Conclusions：DLLM combined with UTMD can inhibit the growth of the cervical lymph node metastatic carcinoma from the macroscopic view.Furthermore,it can inhibit the growth of the cervical lymph node metastatic carcinoma from the molecular level.