脐带间充质干细胞移植治疗大鼠糖尿病

Umbilical cord mesenchymal stem cell transplantation for the treatment of diabetes mellitus in rats

背景:脐血间充质干细胞具有很强的增殖能力和分化能力,在趋向分化作用下可以分化成胰岛β细胞,进而起到治疗糖尿病的作用。目的:观察移植脐带间充质干细胞对大鼠糖尿病的治疗效果。方法:30只雄性SD大鼠中随机取6只作为对照组,注射生理盐水;其中24只按45 mg/kg的剂量注射链脲霉素建立糖尿病模型后,随机等分为移植组和糖尿病组,移植组大鼠尾静脉注射移植脐带间充质干细胞。结果与结论:造模后30 d,糖尿病组大鼠空腹血糖维持在较高水平,且高于对照组(P<0.05)。造模后,与糖尿病组相比,移植组大鼠空腹血糖水平显著下降(P<0.05),体质量显著增加(P<0.01),45 d时移植组大鼠空腹血糖水平与体质量接近对照组水平(P>0.05),而糖尿病组大鼠空腹血糖维持较高水平,且体质量持续下降。提示脐带间充质干细胞移植能有效治疗大鼠糖尿病。

BACKGROUND：Umbilical cord mesenchymal stem cels have strong proliferation and differentiation capacities, and can be induced to differentiate into pancreatic β cels, thereby playing a therapeutic effect on diabetes mel itus. OBJECTIVE：To study the therapeutic effects of transplantation of umbilical cord mesenchymal stem cels for treatment of diabetes melitus in rats. METHODS： Thirty male Sprague-Dawley rats were randomly divided into control group （n=6）, transplantation group （n=12） and diabetic group （n=12）. Rats in the control group were given normal saline injection. Rats in the other two groups were injected with streptozotocin at a dose of 45 mg/kg to establish diabetic models. After modeling, transplantation of umbilical cord mesenchymal stem celsviatail vein was given in the transplantation group. RESULTS AND CONCLUSION：Thirty days after modeling, the fasting blood glucose was maintained at a higher level in comparison with the control group （P 〈 0.05）. However, compared with the diabetic group, the level of fasting blood glucose was significantly reduced （P 〈 0.05） and the body mass was increased in the transplantation group （P 〈 0.05）. After 45 days, the fasting blood glucose level and body mass in the transplantation group had no significant difference from those in the control group （P 〉 0.05）, but in the diabetic group, the fasting blood glucose level was stil higher and the body mass continued to decrease. These findings indicate that the transplantation of umbilical cord mesenchymal stem cels can be effective in the treatment of diabetes melitus in rats.