高龄孕妇胚胎移植前染色体DNA芯片的非整倍体分布分析

The distribution analysis of abnormal aneuploid chromosome of preimplantation embryos by DNA microarray in advanced maternal age women

目的通过对移植前胚胎进行基因芯片检查,筛选出最常发生整倍体变异的染色体,为降低畸形胎儿的怀孕率提供重要依据。方法对219例行胚胎植入的高龄孕妇的移植前胚胎进行基因芯片检查,并对基因芯片中发现非整倍体异常的病例进行FISH杂交验证。结果在异常胚胎里,染色体发生单处异常在16号染色体发生的几率最高,而染色体发生多处异常在15号染色体发生的几率最高。结论 15、16号染色体可能是影响胚胎停育的染色体,15、16号染色体的非整倍体异常可能是导致高龄孕妇早期流产的主要染色体。

Objective In order to reduce the rate of malformation fetus, microarray platform are used for screening preimplantation embryos, most often abnormal chromosomes are detected. Methods 219 case of advanced aged-women were having microarray detection of the aneuploid embryos before embryo implantation and verified by fluorescence in situ hybridization （FISH）. Results In abnormal embryos, the 16 th chromosome shows the highest occurrence of a single place in abnormal chromosome, however, the occurrence of multiple chromosomal abnormalities is highest in chromosome 15. Conclusion Embryos which stop development may be caused by the abnormalities of 15 th, 16 th chromosome, and the aneuploid blastoeyst may be the main reasons to the miscarriage for aged-women.