加替沙星-聚癸二酸酐局部缓释系统预防兔骨髓炎

Gatifloxa-polysebacic anhydride local delivery system for prevention of osteomyelitis in rabbits

背景:聚酸酐材料具有表面溶蚀性、生物可降解性和释药速率可调性,为人体药物缓释材料,但关于聚癸二酸酐缓释系统的应用至今少有报道。目的:观察加替沙星-聚癸二酸酐局部缓释系统对兔股骨骨髓炎感染模型的预防作用。方法:按Norden法将30只兔制作股骨金黄色葡萄球菌骨髓炎兔模型,分别用加替沙星-聚癸二酸酐缓释制剂和加替沙星进行干预,并与模型组作对照,共分3组,每组10只,观察建模后兔一般情况、解剖学观察、X射线片表现、骨培养及细菌计数、组织学检查。结果与结论:建模后8周,模型组兔建模后,食欲差,皮毛不光滑,体质量减轻,3只兔于3周内死亡,其他7只X射线片上骨膜反应较重,可见小块死骨形成,解剖学检查均可见脓肿形成,骨培养及组织学检查均可见骨髓炎表现;加替沙星组建模后体质量减轻,2只兔于建模后3周内死亡,解剖学检查可见有4只动物有脓肿形成,X射线片上可见8只动物骨膜反应较轻,未见死骨形成,骨培养及组织学检查6只兔有骨髓炎表现;加替沙星-聚癸二酸酐缓释制剂治疗组兔一般情况好,解剖学检查未见异常,X射线片检查、骨培养和细菌计数、组织学检查均未见骨髓炎表现。结果证实,加替沙星-聚癸二酸酐局部缓释制剂预防股骨骨髓炎感染效果较好。

BACKGROUND：Polyanhydride materials characterized as surface corrosion resistance, biodegradability and adjustable release rate have been used as drug delivery materials for the body, but the application of polysebacic anhydride delivery system has been rarely reported. OBJECTIVE：To study the role of gatifloxa-polysebacic anhydride local delivery system in the prevention of osteomyelitis in rabbits. METHODS：According to Norden method, we made Staphylococcus aureus-induced osteomyelitis models in the femur of 30 rabbits. There were three groups in the study, 10 rats in each group. Gatifloxa-polysebacic anhydride local delivery system and gatifloxa were respectively used in comparison with model group. After modeling, general observation, anatomical observation, X-ray examination, bone culture and bacteria counting, and histological examination were performed. RESULTS AND CONCLUSION：After 8 weeks, animals in the model group presented with poor appetite, rough fur, and weight loss. Among these 10 rabbits, 3 rabbits died within 3 weeks, and another 7 rabbits appeared to have severe periosteal reactions on the X-ray films, that is, abscess formation shown in the anatomical examination and visible manifestation of osteomyelitis through bone culture and histological examination. In the gatifloxa group, two rabbits died within 3 weeks, abscess formation was found in four rabbits, and periosteal reactions were mild without bone sequestration. Six of 10 rabbits in the gatifloxa appeared to have manifestation of osteomyelitis. In the delivery system group, the animals had a good general state of health and no abnormality was found in the anatomical examination, X-ray examination, bone culture and bacteria count, and histological examination. These findings indicate that the gatifloxa-polysebacic anhydride local sustained release preparation could effectively prevent osteomyelitis.