多肽修饰可促进心血管植入材料的内皮化

Modification of cardiovascular grafts with peptides to promote endothelializaion

背景:将多肽类生物信息分子应用于心血管植入材料的表面修饰,人工模拟细胞外基质功能蛋白,可以促进材料的内皮化。目的:回顾近年来关于多肽修饰在心血管植入材料内皮化方面的相关研究,为相关研究提供新的实验思路。方法:应用计算机检索1990年1月至2013年12月PubMed数据库中有关多肽修饰促进材料内皮化方面的研究,检索关键词为"peptides,surface modification,endothelialization",根据纳入排除标准选择,最终选择43篇文献进行综述。结果与结论:选择来源于细胞外基质功能蛋白的短肽表面修饰各类材料,既能促进内皮细胞的黏附,也能避免直接引入天然细胞外基质成分的缺陷。非特异性多肽主要包括RGD和YIGSR等,此类多肽均能促进包括内皮细胞在内多种细胞类型的黏附。特异性多肽主要包括REDV、CAG和SVVYGLR等,这些多肽可选择性促进内皮细胞的黏附和扩展。应用此类多肽表面修饰,可特异性促进材料的内皮化。联合应用几种多肽或联合特异性多肽与其他一些生物信息分子也许是未来应用多肽修饰促进心血管植入材料内皮化的理想方案。

BACKGROUND：Modification with peptides to mimic the functional proteins of extracellular matrix can promote adhesion and spreading of endothelial cells, and facilitate endothelialization. OBJECTIVE：To review the studies concerning the modification with peptides in the endothelialization of cardiovascular implants in recent years, and to provide the new ideas for relative research. METHODS：The PubMed database was retrieved with computer from January 1990 to December 2013 for the articles on peptides modification to facilitate endothelialization. The key words were“peptides, surface modification, endothelialization”. Final y, 43 articles were included for review according to the inclusion and exclusion criteria. RESULTS AND CONCLUSION：Peptides derived from extracellular matrix proteins are used to modify cardiovascular materials, which can improve the adhesion of endothelial cells, and also avoid the defects by directly employing the natural extracellular matrix components. Nonspecific peptides, including RGD and YIGSR, can promote the adhesion of a variety of celltypes including endothelial cells. Specific peptides, including REDV, CAG and SVVYGLR, can selectively promote endothelial celladhesion and spreading. Surface modification with specific peptides can specifical y promote endothelialization of biomaterials. The combined application of specific peptides with other biological information molecules or several peptides may be the alternative approach to promote endothelialization of cardiovascular implants.