田七口服液对SD大鼠骨折愈合过程中BMP-2和FGF-2表达的影响

Effects of Panax Notoginseng Oral Solution on Expression of BMP-2 and FGF-2 during Fracture Healing of SD Rats

目的:研究田七口服液对SD大鼠桡骨骨折愈合过程中骨痂中骨形态发生蛋白2(BMP-2)和成纤维细胞生长因子2(FGF-2)表达的影响,探讨田七口服液促进骨折愈合的机制。方法:3月龄SD大鼠120只,造成右桡骨骨折后,随机分为田七口服液,阳性药物对照组(接骨七厘片组)、阴性对照组(生理盐水组),分别在实验的第3天、7天、1 4天、21天时,测量骨痂厚度,采用免疫组织化学方法检测BMP-2和FGF-2表达的变化。结果:田七口服液组和阳性药物对照组在骨折后14d BMP-2的表达达到峰值,两者间比较无显著性差异(P>0.05),与阴性对照组比较存在显著性差异(P<0.01)。田七口服液组在骨折后7d FGF-2的表达达到峰值,与阴性对照组比较差异显著(P<0.01),与阳性对照组比较无显著性差异(P>0.05)。结论:田七口服液能增加大鼠骨折端骨痂厚度,在骨愈合早期上调BMP-2和FGF-2的表达,促进骨折愈合。

Objective：To investigate the effects of Panax notoginseng on expression of BMP-2 and FGF-2 in bony callus in the process of radial fracture healing of SD rats’ fracture of tibia,and explored the mechanism of treating fracture with Panax notoginseng Methods：Radial fracture model was set up in SD rat, and 120 SD rats were divided randomly into the Panax notoginseng group,positive control group and negative control group. Selecting 3,7,14 and 21 days in the experiment, measuring the thickness of the callus and the immunohistochemistry method was adopted to detect the change of expression of BMP-2 and FGF-2.Results：Panax notoginseng group appeared as the peak of expression BMP-2 on l4th day after operation, compared with positive control group, significant differences were absent（P〉0.05）,but there were significant differences between Panax notoginseng group and negative control group（P〈0.01）. Panax notoginsen group could make expression of FGF-2 achieve peak value on 7th day after operation, compared to the positive control group：significant differences were absent （P〉0.05）, and significant differences were present comparing to the negative control group（P〈0.01）. Conclusion：In the early of bone repair, Panax notoginseng could promote fracture healing, through up-regulating expression of VEGF and FGF-2 at molecular level and increase the thickness of the callus.