灭多威对美国棉铃虫细胞的抑制作用研究

Acute toxicity effects of drugs on insectcells via microcalorimetry

运用 LKB2 2 77生物活性检测仪在 2 8℃下测定了昆虫细胞 (美国棉铃虫 Hz)的离体基础代谢热值 (其值为 2 3.6p W/ cell) ,研究了不同浓度的灭多威农药对昆虫细胞代谢产热功率的影响 ,并对其影响进行了讨论 .同时测得了农药 (灭多威 )对美国棉铃虫 Hz的半抑制量浓度 EC5 0 为1 96μg·m L- 1 。

This paper developes a microcalorimetric bioassay for acute toxicity based on heat evolution from insect cells,and discusses a reduction in heat production by cells reflects the inhibition of various biochemical reactions which constitute the insect cell metabolic processes.For methomyl,this paper also measures the concentration that is 50 % effective(EC50 ) in inhibiting heat evolution in heliothis zea.