对虾白斑症病毒体内外感染的研究

Studies on infection of white spot syndrome virus in vivo and in vitro

WSSV可经注射、投喂或共居感染克氏螯虾和日本沼虾 ,病毒感染的组织匀浆物注射后发病很快 ,但蔗糖介质纯化的 WSSV注射后发病较慢 ,Ficoll-4 0 0介质纯化的 WSSV注射后发病时间介于前二者之间 .中国对虾细胞内 WSSV一般每个囊膜只含一个核衣壳 ,但此毒种经克氏螯虾反复增殖后 ,多粒包埋的病毒粒子明显增多 .从病虾的每克鳃可提出约 0 .66mg的 WSSV.含WSSV的血淋巴或组织匀浆液感染 8种昆虫细胞后 ,传代

White spot syndrome virus infected Cambarus clarkii and Macrobrachium nipponensis by the means of injection,feeding or co- habitation. The period of this disease was short and the mortality was high.The inoculum was prepared from gill, pereiopod ortail of diseased shrimps by slow centrifugation could cause injected shrimps to die fast,but WSSV purified by sugar of Ficoll- 40 0 cushion centrifugation made shrimps die slowly.Only few viruses whose envelope included two or nucleocapsids existed in cells of Penaeus chinesis,but a lot of viruses whose envelop included two or more nucleocapsids were observed in cells of Cambarusclarkii.The weightof WSSV per gram dried gill was 0 .66mg. The infection of eight insect cells with wssv was not successful.