佛甲草及其近缘种垂盆草的HPLC特征指纹图谱研究

HPLC Characteristic Fingerprints of Sedi Linearis Herba and Sedi Herba

目的:建立不同采收期佛甲草的HPLC特征图谱,并与外形极似的近缘植物药垂盆草进行比较,为其鉴别及质量控制提供可靠的分析方法。方法:用HPLC法研究不同采收期佛甲草的特征图谱及垂盆草的特征图谱,比较异同。结果:从佛甲草HPLC特征图谱中标定了金丝桃苷、异槲皮苷、黄芪苷3个共有峰;相同产地不同生境的佛甲草的HPLC图谱基本一致;同一产地不同采收期佛甲草的HPLC图谱差异显著,多数共有峰峰面积有不同程度变化,且峰的数目(即成分种类)也有所差异。两种植物药HPLC谱图虽有4个共有峰,但有显著区别;研究认为二者均以4月底至5月盛花期时采收为宜。结论:建立的HPLC特征指纹图谱为佛甲草鉴定及质量分析提供了重要参考依据。

Objective： To study HPLC characteristic fingerprint of Sedum lineare from different harvest periods,and to compare with its related species Sedum sarmentosum.Methods： The HPLC fingerprints of Sedum lineare from different collecting periods were established and compared with Sedum sarmentosum by the same detection method.Results： Hyperin,isoquercitrin and astragaloside were identified from the HPLC fingerprint of Sedum lineare.The fingerprint of Sedum lineare growing in the same area but different environment were basically identical; while there were remarkable differences of Sedum lineare growing in the same place but from different harvest periods,with the area of most common peaks changing from little to great,and slightly different peak number.The HPLC fingerprint of the two Sedum species had four common peaks,but could be distinguished from each other.The optimal harvest period of these two species should be full-bloom stage.Conclusion： The established method can provide reference for identification and quality analysis of Sedum lineare.