两种人乳头状瘤病毒检测方法的比较

Comparison of Real-time PCR and Flow Through Hybridization for HPV Detection

目的评价导流杂交和实时荧光定量(real-time)PCR对宫颈癌患者HPV感染检测的临床意义。方法采用导流杂交和real-time PCR分别对190例患者进行HPV感染检测,并对检测结果进行分析。结果在190例检测标本中,导流杂交法检测出113例HPV阳性,阳性检出率为59.5%,筛查宫颈癌的敏感度为58.1%,特异度为39.2%,阳性预测值为47.8%,阴性预测值为49.4%。real-time PCR检测出106例阳性,阳性检出率为55.8%,筛查宫颈癌的敏感度为54.8%,特异度为43.3%,阳性预测值为48.1%,阴性预测值为50.0%。导流杂交和real-time PCR检出的HPV阳性率差异无统计学意义,而且一致性检验显示两种方法具有较高的一致性(符合率为87.9%)。结论本研究结果表明,导流杂交和real-time PCR方法检测HPV未表现出较大的差异,在宫颈癌筛查中可以将两种方法结合使用,以提高检测的准确性,降低漏诊率。

Objective To evaluate the clinic value of flow through hybridization and real-time PCR in detecting HPV of cervical carcinoma.Methods 190 cases of HPV of cervical carcinoma were detected by using flow through hybridization and real-time PCR,respectively.Results There were 113 positive cases （59.5%）detected with flow through hybridization,and the sensitivity,specificity,positive-predictive value and negativepredictive value in detecting HPV for cervical carcinoma were 58.1%,39.2%,47.8% and 49.4% respectively.There were 106 positive cases （55.8%） detected with real-time PCR,and the sensitivity,specificity,positive-predictive value and negative-predictive value in detecting HPV for cervical carcinoma were 54.8%,43.3%,48.1% and 50.0% respectively.There was a concordance rate of 87.9% in the detection of HPV between two methods.Conclusion The results of this study suggest that there are no significant differences in the detection of HPV between flow through hybridization and real-time PCR.The detective rate may be improved in screening cervical carcinoma by combination of the two methods.