低温诱导红豆杉细胞同步化的研究

Study on the relation between lowtemperature and Taxul cell mitotic index

研究了用低温处理使悬浮培养红豆杉细胞同步化 ,比较了 2℃、4℃、6℃、8℃不同的温度 ,1 2 h、2 4h、3 6h不同的处理时间以及 1 2 h、2 4 h、3 6h不同的恢复时间对红豆杉细胞分裂指数的影响 .结果表明 :2℃处理 1 2 h,恢复 1 2 h,红豆杉细胞分裂指数能达到最高 8.62 % ,提高了 1 1 5%

The synchronization of Taxus chinensis suspension cells were studied by application of low temperature, comparing different temperatures, pretreated time and subculture time which effect the mitotic index.It is suggested that when the cells are pretreated at 2 ℃ for 12h and subcultured for 12h,the ultimate mitotic index could reach 8.62 %, which has increased 115 %.