大鼠脊髓损伤后神经源性膀胱模型的制备

Neurogenic bladder following spinal cord injury:a rat model

目的:提供一套较系统、可重复率高、可变系数低的神经源性膀胱模型的制备方案。方法:采用Hassan Shaker脊髓横断法并加以改良,在Hassan Shaker法的基础上改用国内动物造模常用的水合氯醛行腹腔麻醉,用牙科钩沿横断椎间隙横向钩出脊髓,手术刀切断脊髓后反复刮扫以确定脊髓完全横断;并将此法运用于不同节段的大鼠脊髓损伤模型的制作,根据骶上和骶髓的分组选择不同的位置(T10/11和L2/3椎间隙)进行手术完全切断脊髓,并配合相应的术前、术后护理方案。结果:改良后的造模方案提高了造模后大鼠的存活率(骶上组80%,骶髓组90%)、成模率(骶上组70%,骶髓组80%)并降低了并发症的发生率(骶上组70%,骶髓组60%);造模后大鼠最大膀胱容量较空白组明显升高(P<0.01),与脊髓休克期相比,骶上组大鼠休克期后的最大膀胱容量明显降低(P<0.01),骶髓组大鼠容量较前无明显变化(P>0.05),脊髓休克期后骶髓组大鼠最大膀胱容量明显高于骶上组(P<0.01);经光镜观察,造模后大鼠的膀胱组织形态均发生了不同程度的病理损害。结论:采用改良Hassan Shaker法可制备大鼠骶上和骶髓不同节段脊髓损伤后神经源性膀胱模型。

Objective：Developing a consistency,reliability,controllable and reproducibility method to construct neurogenic bladder and urethra dysfunction rat model following spinal cord injury（SCI）.Method：The surgical procedure and post-operative care was induced by using modified Hassan Shaker method：The intraperitoneal anesthesia was induced by using chloral hydrate and complete sections of spinal cord were performed at the thoracic （T10/11） and lumbar （L2/3） levels.Result：The modified surgical procedure and post-operative care could improve the survival rate （suprasacral spinal cord injury group 80％,sacral spinal cord injury group 90％）,molded rate （suprasacral group 70％,sacral group 80％） and reduced the complications rate （suprasacral group 70％,sacral group 60％）.The maximum bladder volume was significantly higher after being molded（P＜0.01）.After spinal cord shock,maximum bladder volume was significantly less in suprasacral spinal cord injury group than that during the shock（P＜0.01）,but there was no significant difference in the sacral spinal cord injury group.After spinal cord shock,average bladder volume was significantly less in suprasacral injury group than that in sacral injury group（P＜0.01）.Conclusion：The modified Hassan Shaker method was suitable for the construction of neurogenic bladder and urethra dysfunction rat model following spinal cord injury.