参附注射液对前列腺癌PC-3细胞凋亡诱导的影响

Shenfu Injection induces the apoptosis of prostate cancer PC-3 cells

目的:研究参附注射液(SF)对人前列腺癌PC-3细胞凋亡的影响及可能机制。方法:实验设立对照组和SF 50、100、200μl/ml组,Annexin V/PI染色流式细胞术检测细胞凋亡,RT-PCR检测p53 mRNA表达。结果:与对照组比较,作用24、48、72 h后,SF 50、100、200μl/ml组PC-3细胞存活率显著减少(P均<0.05)。SF各组24 h存活率分别为(93.76±2.63)%、(81.21±1.80)%、(18.01±3.84)%;48 h存活率分别为(94.67±1.11)%、(78.33±2.89)%、(10.34±1.44)%;72 h存活率分别为(91.30±0.47)%、(36.67±1.56)%、(1.33±0.32)%,呈浓度和时间依赖性。作用48 h后,p53 mRNA表达明显升高(P<0.05)。结论:SF可以诱导PC-3细胞凋亡,其作用机制可能与p53表达增高相关。

Objective ： To study the effect of Shenfu Injection （SF） on the apoptosis of prostate cancer PC-3 cells and its possibale mechanism. Methods ： We divided prostate cancer PC-3 cells into a blank control group and three experimental groups, the latter treated with SF at 50, 100, and 200 μl/ml, respectively, for 24, 48, and 72 hours. Then we determined the proliferation of the cells by MTT assay, measured their apoptosis by Annexin V/PI flow cytometry, and detected the expression of P53 mRNA by RT- qPCR. Results： Compared with the blank control group, the survival rates of the prostate cancer PC-3 cells in the 50, 100, and 200 μl/ml SF groups were （93.76 ±2.63）%, （81.21 ±1.80）% and （18.01 ±3.84）% at24 hours, （94.67 ±1. 11）%, （78.33 ± 2.89 ） % and （ 10.34 ± 1.44 ） % at 48 hours, and （91.30 ±0. 47） %, （36. 67 ± 1.56） % and （ 1.33 ± 0.32） % at 72 hours, all significantly increased in a dose- and time-dependent manner （P 〈 0.05）. The expression of p53 mRNA was also maikedly increased in all the three experimental groups at 48 hottrs （ P 〈 0.05 ）. Conclusion： SF can inhibit the proliferation and induce the apeptosis of PC-3 cells, which may due to its upregulation of the p53 mRNA expression.