结直肠癌中L-选择素表达及其与淋巴转移的关系

Expression of L-selectin in colorectal carcinoma and its relation with lymphatic metastasis

目的探讨L-选择素(L-selectin)在结直肠癌组织中的表达及其与结直肠癌淋巴转移的关系。方法采用免疫组化PV9000两步法检测104例结直肠癌组织、55例正常结直肠黏膜组织中L-selectin的表达。应用流式细胞术(flow cytometry,FCM)定量检测45例结直肠癌组织和17例正常结直肠黏膜组织中L-selectin的含量。结果结直肠癌组织中L-selectin阳性率及表达量均明显高于癌旁正常黏膜组(P<0.05);有淋巴转移组明显高于无转移组(P<0.05);TNM分期中Ⅲ+Ⅳ期组明显高于Ⅰ+Ⅱ期组(P<0.05)。结论 L-selectin在结直肠癌中的表达与结直肠癌的发生、TNM分期、淋巴结转移有关,其可能参与结直肠癌发生及淋巴结转移过程。

Purpose To investigate the expression of adhesion molecule L-selectin in human colorectal carcinoma, and to analyze their correlation with lymph node metastasis. Methods The expression of L-selectin was detected by immunohistochemical method in 104 cases of colorectal carcinoma and 55 cases of normal intestinal mucosa adjacent to carcinoma. Flow cytometry （FCM） was used to quantify the contents of L-selectin in 45 cases of colorectal carcinoma and 17 cases normal intestinal mucosa. Results The positive rate and quantified expression of L-selectin in the colorectal carcinoma group were significantly higher than that in adjacent mucosa group （P 〈 0. 05）. The expression in the tumor with lymph node metastasis was significantly higher than that in the tissue without lymph node metastasis （P 〈 0.05）. As for TNM stage, the expression in stage Ⅲ＋ Ⅳ group was significantly higher than that in stage Ⅰ ＋ Ⅱ group （P 〈 0. 05）. Conclusions The expression of L-selectin is closely associated with development, TNM stage and metastasis of colorectal carcinoma. It may participate in the carcinogenesis and lymph node metastasis of colorectal carcinoma.