补肾复方对慢性疲劳综合征线虫模型的影响

Effect of Compound Bushen Recipe on Chronic Fatigue Syndrome inC. elegans : an Experimental Study

目的以各种应激状态在线虫上模拟应激疲劳综合征(chronic fatigue syndrome,CFS),评估补肾复方对成年线虫应激生存状态及整体寿命的影响。方法在高、中、低浓度(500、250、100 mg/L)补肾复方干预下,观察成年线虫对体外环境的热应激(35℃)、氧化应激(250μg/mL)及体内毒性蛋白应激(Aβ1-42转基因突变株CL4176)的耐受能力和平均存活时间,评估补肾复方对正常状态下线虫平均生存时间(25℃)、20s运动距离、30 s咽泵运动频次及生殖能力的作用效果。结果与对照组比较,补肾复方干预各组均能增加线虫热应激存活时间(P<0.01);高、中浓度干预组可增加线虫氧化应激存活时间,减轻蛋白毒性作用,延长线虫生存时间(P<0.01,P<0.05);高浓度干预组亦能增加线虫的运动距离和咽泵运动频次(P<0.01)。各组间生殖能力比较,差异无统计学意义(P>0.05)。结论补肾复方能增强线虫对内外环境压力的应激能力,延长线虫寿命的同时不干扰线虫正常生殖,提高生命质量,为补肾复方在防治CFS方面的进一步机制探索及药理研究奠定基础。

Objective To evaluate the effect of Compound Bushen Recipe （CBR） in improving the survival state of stress and the overall life span in C. elegans by simulating chronic fatigue syndrome （CFS） under various stress states. Methods The tolerance and the average survival time of adult larvae against heat stress （35℃）, oxidative stress （250μg/mL juglone）, and in vivo Aβ protein toxicity （Aβ1-42 transgenic mutant CL4176） under the intervention of the high （500 mg/L）, middle （250 mg/L）, and low （100 mg/L） dose CBR were observed. The effect of CBR on the average live time （at 25℃）, movement distance in 20 seconds, the frequency of pharyngeal pump in 30 seconds, and the reproductive capability were assessed. Results Compared with the control group, the survival time of heat stressed C. elegans could be significantly increased in each CBR group （P 〈0. 01 ）. The survival time of heat stressed C. elegans could be elongated, the protein toxicity be attenuated, and the live time prolonged in the high and middle dose CBR groups （P 〈0. 01, P 〈 0. 05）. The movement distance and the frequency of pharyngeal pump could also be increased in the high dose CBR group （P 〈0. 01 ）. There was no statistical difference in the reproductive capability among all groups （P 〉 0. 05）. Conclusions CBR could significantly enhance the stress capacity of C. elegans against internal and external environment, and prolong their lifespan. It did not interfere their normal production, and also could improve the quality of life, thus laying a foundation for further mechanism studies and pharmacological researches on CBR in preventing and treating CFS.