细粒棘球蚴抗原B对小鼠早期实验性1型糖尿病的影响

Effect of echinococcus granulosus antigen B on streptozotocin induced diabetes mellitus in mice

目的 探讨细粒棘球蚴抗原B对小鼠1型糖尿病发病的早期影响及其机制.方法 30只BALB/c小鼠随机分组：细粒棘球蚴抗原B＋糖尿病组（A组）;生理盐水＋糖尿病组（B组）;糖尿病组（C组）;A组给予细粒棘球蚴抗原B,B组给予生理盐水,随后A、B、C组均给予小剂量链脲佐菌素诱导1型糖尿病.观察血糖变化,留取标本进行细胞因子等指标测定.结果 在糖尿病成模后3周,A组＜40％的胰岛可见淋巴细胞浸润,B组和C组有80％的胰岛见淋巴细胞浸润,差异有统计学意义（A组、B组和C组的平均Ridit值分别为：0.423、0.519、0.561,P＜0.05）;糖尿病模型建立3周末,A组小鼠血清白细胞介素-4（IL-4）水平高于B组和C组[（71.6±12.4） ng/ml比（12.6±5.6） ng/ml和（14.2-7.2） ng/ml,P＜0.05],A组小鼠血清γ-干扰素（IFN-γ）水平低于B组和C组[（276.1 ±41.7） ng/ml比（352.2±52.2）ng/ml和（358.1 ±53.4） ng/ml,P＜0.05]. 结论 细粒棘球蚴抗原B对小鼠1型糖尿病早期胰岛细胞的自身免疫损害具有拮抗作用,其机制可能与Th1/Th2免疫偏移有关.

Objective To investigate the effect of echinococcus granulosus antigen B on the severity of streptozotocin induced diabetes mellitus.Methods Thirty male BALB/c mice were randomly divided into 3 groups：echinococcus granulosus antigen B group （group A,n =10）,normal saline group （group B,n =10）,control group （group C,n =10）.Mouse in group A was injected by echinococcus granulosus antigen B and mouse in group B was given normal saline,Type 1 diabetes was induced.After 3 weeks,mice were executed and pancreases were scored on insulitis by HE staining.Serum IFN-γ and IL-4 levels were measured by ELISA.Results After 3 weeks of the establishment of diabetes model mouse body weight in group B and C decreased significantly compared with that in group A.Mouse mean blood glucose level in group A was significantly lower than that in group B and C.There were less than 40％ of islets with lymphocytic infiltration in group A,compared with 80％ in group B.The average Ridit was 0.423,0.519,and 0.561 in group A,B and C respectively,P ＜ 0.05.IL-4 level in group A was significantly higher than that in group B and group C [（71.6 ± 12.4） ng/ml,（12.6 ± 5.6） ng/ml,（14.2 ± 7.2） ng/ml,P ＜ 0.05].IFN-γ level in group B and group C were higher than that in group A [（276.1 ± 41.7） ng/ml,（352.2 ± 52.2） ng/ml,（358.1 ± 53.4） ng/ml,P ＜ 0.05].Conclusions Type 1 diabetes is organ specific T lymphocyte mediated autoimmune disease.Echinococcus granulosus antigen B has protective effects on diabetes mellitus in mice couteracting autoimmune injury to the islets by streptozotocin,probably by a mechanism related to immune deviation of Th1 to Th2.