HLA-G在子痫前期患者血清及胎盘中的表达及意义

Analysis of HLA-G Expression in the Serum and Placenta of Preeclampsia Patients

目的:探讨人类白细胞抗原G(HLA-G)在子痫前期患者血清及胎盘组织中的表达及意义。方法:收集子痫前期患者20例(观察组)和正常妊娠者20例(对照组)的血清及胎盘组织,采用酶联免疫吸附法(ELSIA)、实时荧光定量PCR(real-time RT-PCR)以及免疫蛋白印迹(Westen Blot)检测两组血清可溶性人类白细胞抗原G(sHLA-G)及胎盘组织中HLA-G的表达差异,并通过HE染色观察两组胎盘的病理变化。结果:①观察组血清及胎盘组织中HLA-G表达水平均低于对照组,两组差异均有统计学意义(P<0.05)。②观察组与对照组比较,胎盘绒毛间质血管数及绒毛数平均值减少(P<0.01,P<0.05),合体滋养细胞结节增多(P<0.05),绒毛间质纤维化及纤维素样坏死显著增多(P<0.01),细胞滋养细胞明显增生(P<0.01),干绒毛小动脉管腔直径明显变小(P<0.01)。③相关分析显示,血清中sHLA-G表达水平与胎盘中HLA-G蛋白表达水平呈正相关(r=0.909,P<0.05)。HLA-G mRNA及HLA-G蛋白表达强度与胎盘绒毛血管密度呈正相关(r=0.907,P<0.05;r=0.904,P<0.05)。结论:HLA-G可能与子痫前期的发生、病理生理过程密切相关。

Objective： Explore the expression of human leukocyte antigen-G （HLA-G）in the serum and placenta of preeclampsia patients. Methods： Enzyme-linked immunosorbent assay （ ELISA）, real-time PCR and Western Blotting were used to detect HLA-G expression in serum and placenta tissue in 20 patients with preeclampsia （ experimental group） and 20 normal pregnant women （ control group）. Pathological characteristics of placenta tissue was also compared between two groups. Results：（1） HLA-G in both serum and placenta decreased significantly in the preeclampsia group （P 〈 0. 05）. （2）Compared with normal pregnant women,the amount of placenta villus stroma blood vessels （ P 〈 0. 01 ） and villus （ P 〈 0.05） decreased in patients with preeclampsia, which was characterized by increase of syneytiotrophoblast cells nodule（ P 〈 0.05）, increase of villus stroma fibrosis and fibrionoid necrosis （ P 〈 0. 01 ）, increase of cytotrophoblastic cells （ P 〈 0.01 ）, and decrease of villus arteride diameter（ P 〈 0. 01 ）. （3）There was a positive correlation between ser- um and placental HLA-G levels（ r =0. 909, P 〈0.05）. There was a positive correlation of the expression of either HLA-G mRNA or protein with the count of vessels in placental villus（ r=0. 907 and 0. 904, P〈0. 05）. Conclusions：The expression of HLA-G decreased significantly in serum and placental tissue of preeclampsia patients,which may be correlated with the physiological process of preeclampsia.