吸烟促进牙周炎牙槽骨的吸收效应

Smoking promotes the resorption of periodontal alveolar bone

背景:多项研究证实吸烟能够促进牙周炎性牙槽骨吸收。目的:观察吸烟在牙周炎性牙槽骨吸收大鼠中的作用。方法:24只大鼠随机分为3组,正常组:正常饲养,不给予其他处理;对照组:采用钢丝结扎法建立大鼠实验性牙周炎动物模型;实验组:在造模期间给予被动吸烟。8周后将所有大鼠处死,取出牙周组织,利用苏木精-伊红染色观察牙周组织的病理学改变,运用免疫组织化学染色观察核因子活化因子受体配体和骨保护素的表达变化。结果与结论:对照组和实验组大鼠造模8周后,实验组大鼠牙槽骨中核因子κB受体活化因子配体表达量高于对照组(P<0.05),而对照组大鼠牙槽骨中骨保护素的表达量较实验组有明显上调(P<0.05)。提示吸烟能够提高牙周炎大鼠核因子κB受体活化因子配体的表达,同时并可以降低骨保护素的表达变化,在一定程度上加重了牙周炎性牙槽骨的吸收。

BACKGROUND：Various studies confirm that smoking can contribute to the resorption of periodontal alveolar bone. OBJECTIVE：To observe the effect of smoking in alveolar bone resorption of periodontitis rat models. METHODS：A total of 24 rats were randomly divided into three groups. Normal group：rats were normal y fed without any other pre-treatment;Control group：experimental periodontitis model was established using wire ligation method in rats;Experimental group：rat models were given passive smoking during experimental modeling period. Al rats were sacrificed after 8 weeks of modeling, periodontal tissue were removed. Hematoxylin-eosin staining was used for examining pathological changes in periodontal tissue, and immunohistochemical analysis was done for observing receptor activator of nuclear factor-κB ligand and osteoprotegrin expression. RESULTS AND CONCLUSION：After 8 weeks of modeling, expression of receptor activator of nuclear factor-κB ligand factor was significantly higher in alveolar bone of rats from experimental group in comparison to control group （P〈0.05）, whereas expression of osteoprotegerin in alveolar bone was significantly greater in rats from control group when compared to experimental group （P〈0.05）. This finding suggests that smoking can increase the expression of receptor activator of nuclear factor-κB ligand protein and reduce osteoprotegrin expression in periodontal rats, thus increasing the resorption of periodontal alveolar bone.