益肾保真方对肾性贫血大鼠血清促红细胞生成素表达的影响

Effect of Yishenbaozhen Recipe on serum erythropoietin level in renal anemia rats

目的探讨益肾保真方对慢性肾衰竭大鼠贫血的干预作用及疗效机制。方法采用二步5/6肾切除的方法复制慢性肾衰竭大鼠贫血模型,将造模成功的43只大鼠随机分为正常组(n=9)、模型组(n=8)、益肾保真方高剂量组[n=9,46.5g/(kg·d)]、益肾保真方低剂量组[n=9,15.6g/(kg·d)]及尿毒清组[n=8,2.5g/(kg·d)]。灌胃给药,每日1次,连续6周,测定各组大鼠血肌酐(SCr)、尿素氮(BUN)、血红蛋白(Hb)、红细胞计数(RBC)、平均红细胞容积(MCV)及血清促红细胞生成素(EPO)含量。结果益肾保真方高、低剂量组Hb、RBC及EPO水平均较模型组和尿毒清组升高(P<0.05),MCV在组间的差异无统计学意义;与模型组比较,益肾保真方高、低剂量组BUN、SCr水平均降低(P<0.01),但与尿毒清组比较差异无统计学意义。结论益肾保真方对肾性贫血有干预作用,其机制可能与降低氮质潴留、减轻毒素对造血的抑制、提高红细胞刺激因子EPO水平有关。

Objective To explore the intervention effect of Yishenbaozhen Recipe （YSBZR） on the renal anemia in rats with chronic renal failure and the related mechanism. Methods Totally 43 rat models of chronic renal failure were produced by two-step 5/6 nephrectomy. The study was randomly divided into 5 groups： normal control group （n=9）, model group （n=8）, YSBZR high-dose group （n=9, 46.5 g/[kg· d]）, YSBZR low-dose group （n=9, 15.6 g/[kg · d]） and Niaoduqing group （n=8, 2.5 g/[kg · d]）. YSBZR was given intragastrically on a daily bases for 6 weeks, and then the serum erythropoietin （EPO）, serum creatinine （SCr）, blood urea nitrogen （BUN）, hemoglobin （Hb）, red blood cell count （RBC）, and mean corpuscular volume （MCV） were examined. Results Compared with the model group and Niaoduqing group, YSBZR highdose group and low-dose group had significantly higher Hb, RBC and EPO levels （P〈0.05）, and had similar MCV levels. YSBZR high-dose and low-dose groups had significantly decreased SCr and BUN levels compared with the model group （P〈0.01）, and had similar levels when compared with Niaoduqing group. Conclusion YSBZR has interventional effect on renal anemia, which might be related to decreasing nitrogen retention, reducing the toxin-induced inhibition of hematopoietic system, and increasing EPO level.