多重PCR鉴别我国南疆3种常见蛉种

Identification of Three Common Sandfies in Southern Xinjiang with Multiplex PCR

分别于我国南疆地区捕获长管白蛉64只、吴氏白蛉66只和亚历山大白蛉20只。本研究基于COⅠ基因序列差异,建立了鉴别我国南疆3种常见蛉种的多重PCR。结果显示,长管白蛉、吴氏白蛉和亚历山大白蛉的PCR产物分别为248、632和395 bp,且多重PCR结果与形态学鉴定结果完全一致。

Based on the variable part of mtDNA CO I gene sequence, a multiplex PCR method was developed for the identification of the three common sandilies （Phlebotomus longiductus, Ph. wui, and Ph. alexandri） in southern Xinjiang. The results demonstrated that this multiplex PCR method was reliable, and could be used to identify the three Phlebotomus species. The PCR product of CO I gene from Ph. longiductus, Ph. wui and Ph. alexandri was 248, 632, and 395 bp, respectively.