广西在校大学生人芽囊原虫分离株基因型的初步研究

PCR-based Genotype Classification of Blastocystis hominis Isolates from College Students of Guangxi

为了解广西在校大学生人芽囊原虫分离株基因型构成,于2012年收集53例广西医科大学人芽囊原虫感染者粪便,分离虫体,经体外培养后抽提基因组DNA,用已知的7对STS引物进行PCR扩增。结果显示,53株人芽囊原虫分离株中共发现5种已知基因型。其中,1型(351 bp)4株,占7.6%;3型(526 bp)17株,占32.1%;4型(338 bp)4株,占7.6%;6型(317 bp)1株,占1.9%;7型(487 bp)5株,占9.4%。未见基因2型、5型和混合基因型。22株无特异性条带出现,初步认定为未知基因型。

Fifty-three Blastocystis hominis isolates were separated from the fecal specimens of carriers in college students from Guangxi and cultivated in vitro, and the genetic DNA was extracted. All the isolates were genotyped by PCR using seven pairs of known sequence-tagged site （STS） primers. The results showed there were five subtypes in the 53 isolates. Subtype 3 was the most popular one （32.1%, 17/53）, followed by subtype 7 （9.4%, 5/53）. Subtypes 1 （7.6%, 4/53）, 4 （7.6%, 4/53）, and 6 （1.9%, 1/53） were detected, while subtypes 2 and 5 were not detected. The genotypes of the other 22 isolates were unknown which were negative to all the STS primers.