摘要
通过全基因合成猪带绦虫TSOL18基因,将该基因定向克隆至大肠埃希菌-双歧杆菌穿梭表达载体pGEX-1λT中,构建重组质粒pGEX-TSOL18,电穿孔法将该质粒导入长双歧杆菌(Bifidobacterium longum),构建猪带绦虫双歧杆菌表达系统pGEX-TSOL18/B.longum,并通过酶切、PCR和测序鉴定。全基因合成了393 bp的TSOL18基因片段。酶切、PCR和测序鉴定结果证明猪带绦虫双歧杆菌表达系统pGEX-TSOL18/B.longum构建成功。
The TSOL18 gene of Taenia solium was synthesized and cloned into Escherichia coli-Bifidobacteria shuttle vector pGEX-1λT. The recombinant plasmid pGEX-TSOL18 was transformed into Bifidobacterium longum with electroporation. The recombinant plasmid containing TSOL18 gene was identified by restriction endonuclease analysis, PCR and DNA sequencing. The length of synthesized TSOL18 gene was 393 bp. The results indicated that the Bifidobacteria expression system pGEX-TSOL18/B. longum was successfully constructed.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2014年第3期239-241,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家自然科学基金(No.81160206)
遵义医学院博士启动基金(No.ZMKD2013-016)~~