TLR4对MHV-3诱导的C3H/He小鼠病毒性肝炎转归的影响

The Effect of TLR4 on the outcomes of MHV-3 induced viral hepatitis for C3H/He mice

目的探讨TLR4对3型鼠肝炎病毒(MHV-3)诱导的C3H/He小鼠肝炎转归的影响。方法观察C3H/HeJ及C3H/HeN小鼠感染MHV-3后的临床症状(皮毛异常、呼吸加快及身体颤抖)并进行评分。记录两亚系小鼠的存活情况并进行比较。所有小鼠观察至感染后40d。结果小鼠品系对临床症状评分不起作用(P=0.718)。临床评分有随时间变化的趋势(P<0.001),且时间因素的作用随小鼠品系而不同(P=0.004)。C3H/HeJ及C3H/HeN小鼠临床症状评分仅在感染后第5、6、13天出现差异:(13.071±1.184)和(10.933±4.608)(P<0.001),(8.321±5.048)和(11.304±3.901)(P<0.001),(13.091±1.578)和(10.846±3.671)(P=0.015)。C3H/HeJ及C3H/HeN小鼠存活率分别为26.7%和23.3%,平均存活时间分别为16.267d和16.433d,无显著差异(P=0.922)。结论 MHV-3诱导的C3H/He小鼠病毒性肝炎转归不依赖于TLR4。

Objective To explore the effect of TLR4 on the outcomes of mouse hepatitis virus-3 （MHV-3） induced hepatitis for C3H/He mice. Methods Clinical symptoms （abnormalities of skin and fur, accelerated breathing and tremor） of C3H/HeJ mice and C3H/HeN mice infected with MHV-3 were observed and scored. Survival of the two mice substrains was recorded and compared. The observation of all mice lasted for 40 days after the infection. Results Different mice substrains did not affect the scores of clinical symptoms （P=0.718）. The scores varied according to time （P 〈 0.001）, and the effect of time varied according to different mice substrains （P=0.004）. The scores of clinical symptoms in C3H/HeJ mice and C3H/HeN mice only differed on the fifth, sixth and 13th day of the infection： （13.071 ± 1.184） and （10.933 ± 4.608）（P 〈 0.001）, （8.321 ± 5.048） and （11.304 ± 3.901） （P 〈 0.001）, （13.091 ± 1.578） and （10.846±3.671）（P=0.015） respectively. The survival rate of C3H/HeJ mice and C3H/HeN mice was 26.7% and 23.3% respectively, the average survival time was 16.267 days and 16.433 days, and there was no significant difference （P=0.922）. Conclusion Outcomes of MHV-3 induced viral hepatitis for C3H/He mice is not affected by TLR4.